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平臺編號：bio-68314
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國際編號：TIB-39?
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細胞信息： EL4
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
EL4
細胞類型：T淋巴細胞
是否是腫瘤細胞：0
物種來源：小鼠
品系：C57BL/6N
ATCC Number：TIB-39?
相關(guān)疾病：淋巴瘤
運輸方式：凍存運輸
數(shù)量：大量
生長狀態(tài)：懸浮生長
細胞形態(tài)：淋巴樣
規(guī)格：1.0ml Designations: EL4
Depositors: ?M Cohn
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: C57BL/6N
Cell Type: T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host (Roche Transfection Reagents )
Antigen Expression:H-2b; Thy-1.2
Cytogenetic Analysis:modal number = 39
Comments:EL4 was established from a lymphoma induced in a C57BL mouse by 9,10-dimethyl-1,2-benzanthracene. [22448 ]
The cells are resistant to 0.1 mM cortisol and sensitive to 20 mcg/ml PHA.
A subline (EL4.IL-2, ATCC TIB-181 ) that produces high levels of interleukin-2 (IL-2, interleukin 2) is available.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
derivative:ATCC CRL-2113
derivative:ATCC TIB-181
derivative:ATCC TIB-40
References: 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304
22422: Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252
22448: Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344
22523: Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883
22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714
23443: Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916
32256: Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636
32286: Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019
是否是腫瘤細胞：0
物種來源：小鼠
品系：C57BL/6N
ATCC Number：TIB-39?
相關(guān)疾病：淋巴瘤
運輸方式：凍存運輸
數(shù)量：大量
生長狀態(tài)：懸浮生長
細胞形態(tài)：淋巴樣
規(guī)格：1.0ml Designations: EL4
Depositors: ?M Cohn
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: lymphoma
Strain: C57BL/6N
Cell Type: T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host (Roche Transfection Reagents )
Antigen Expression:H-2b; Thy-1.2
Cytogenetic Analysis:modal number = 39
Comments:EL4 was established from a lymphoma induced in a C57BL mouse by 9,10-dimethyl-1,2-benzanthracene. [22448 ]
The cells are resistant to 0.1 mM cortisol and sensitive to 20 mcg/ml PHA.
A subline (EL4.IL-2, ATCC TIB-181 ) that produces high levels of interleukin-2 (IL-2, interleukin 2) is available.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
derivative:ATCC CRL-2113
derivative:ATCC TIB-181
derivative:ATCC TIB-40
References: 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304
22422: Old LJ, et al. The G (Gross) leukemia antigen. Cancer Res. 25: 813-819, 1965. PubMed: 4284252
22448: Gorer PA. Studies in antibody response of mice to tumour inoculation. Br. J. Cancer 4: 372-379, 1950. PubMed: 14801344
22523: Herberman RB. Serological analysis of cell surface antigens of tumors induced by murine leukemia virus. J. Natl. Cancer Inst. 48: 265-271, 1972. PubMed: 4119883
22528: Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714
23443: Shevach EM, et al. Immunoglobulin and theta-bearing murine leukemias and lymphomas. J. Immunol. 108: 1146-1151, 1972. PubMed: 4112916
32256: Aparicio CL, et al. Correction for label leakage in fluorimetric assays of cell adhesion. BioTechniques 23: 1056-1060, 1997. PubMed: 9421636
32286: Cuthbert JA, Lipsky PE. Regulation of proliferation and Ras localization in transformed cells by products of mevalonate metabolism. Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019
