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生長狀態(tài)：懸浮生長
細胞形態(tài)：淋巴樣
免疫類型：IgG1; kappa light chain
運輸方式：凍存運輸
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
ATCC Number：HB-127?
規(guī)格：0.1ml Designations: CE9H9
Isotype: IgG1; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against insulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with zinc containing crystalline human insulin.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to insulin from human, cow, pig and rabbit and to pro-insulin from cow and pig.
The antibody cross-reacts with insulin from sheep, guinea pig, chicken and fish.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 981: Schroer JA, et al. Mapping epitopes on the insulin molecule using monoclonal antibodies. Eur. J. Immunol. 13: 693-700, 1983. PubMed: 6193965