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平臺(tái)編號(hào)：bio-68331
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國(guó)際編號(hào)：HTB-57?
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細(xì)胞信息： SK-LU-1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
SK-LU-1
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
年限：60 years
細(xì)胞形態(tài)：上皮樣
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
ATCC Number：HTB-57?
器官來(lái)源：肺
相關(guān)疾病：腺癌
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
規(guī)格：1mg Designations: SK-LU-1
Depositors: ?G Trempe, LJ Old
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: lung
Disease: adenocarcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Tumorigenic:Yes
Antigen Expression:Blood Type O; Rh+; HLA Aw24, Aw32, B27, Bw41
DNA Profile (STR):Amelogenin: X
CSF1PO: 10
D13S317: 10
D16S539: 8
D5S818: 11
D7S820: 9
THO1: 7
TPOX: 8,10
vWA: 16,17
Cytogenetic Analysis:The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q;11q); 11q+; t(13;?); 16q+; t(12q; 18q); M10; t(2q;13q); i(15); and ?t(xp;21q) occurred in all S metaphases, and t(1p;?); t(1p;14q); t(16;?), and t(14;21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, X chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation.
Isoenzymes: AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 2
PGM3, 1
Age: 60 years
Gender: female
Ethnicity: Caucasian
Comments:Mycoplasma contamination was detected and eliminated in 1971.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
物種來(lái)源：人
年限：60 years
細(xì)胞形態(tài)：上皮樣
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
ATCC Number：HTB-57?
器官來(lái)源：肺
相關(guān)疾病：腺癌
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
規(guī)格：1mg Designations: SK-LU-1
Depositors: ?G Trempe, LJ Old
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: lung
Disease: adenocarcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Tumorigenic:Yes
Antigen Expression:Blood Type O; Rh+; HLA Aw24, Aw32, B27, Bw41
DNA Profile (STR):Amelogenin: X
CSF1PO: 10
D13S317: 10
D16S539: 8
D5S818: 11
D7S820: 9
THO1: 7
TPOX: 8,10
vWA: 16,17
Cytogenetic Analysis:The stemline chromosome number is hypotetraploid, with the 2S component occurring at 4.4%. Marker chromosomes 1p, t(1q;11q); 11q+; t(13;?); 16q+; t(12q; 18q); M10; t(2q;13q); i(15); and ?t(xp;21q) occurred in all S metaphases, and t(1p;?); t(1p;14q); t(16;?), and t(14;21) occurred in some. In addition, 4 to 9 small markers of unidentifiable origin occurred frequently. Chromosome No. 7 was generally hexasomic, X chromosomes were disomic, and normal No. 15 was absent. No Y chromosome was detected in the QM stained preparation.
Isoenzymes: AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 2
PGM3, 1
Age: 60 years
Gender: female
Ethnicity: Caucasian
Comments:Mycoplasma contamination was detected and eliminated in 1971.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212
