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平臺(tái)編號(hào)：bio-68335
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國(guó)際編號(hào)：CRL-1476?
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細(xì)胞信息： A-10
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
A-10
年限：embryo
ATCC Number：CRL-1476?
數(shù)量：大量
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：DB1X
組織來源：medial layer
是否是腫瘤細(xì)胞：0
物種來源：大鼠
運(yùn)輸方式：凍存運(yùn)輸
器官來源：其他
細(xì)胞形態(tài)：成肌細(xì)胞
規(guī)格：0.2ml Designations: A-10
Depositors: ?W Carlisle
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:myoblast
Source: Organ: aorta, thoracic
Strain: DB1X
Tissue: medial layer
Cellular Products:myokinase; creatine phosphokinase; myosin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Age: embryo
Comments:The clonal cell line A10 was derived by B. Kimes and B. Brandt from the thoracic aorta of DB1X embryonic rat and possesses many of the properties characteristic of smooth muscle cells.
The cells produce spontaneous action potentials at the stationary phase of the growth cycle and exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 29 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1061: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301
32281: Zhang X, et al. Microfilament depletion and circumvention of multiple drug resistance by sphinxolides. Cancer Res. 57: 3751-3758, 1997. PubMed: 9288783
32468: Gordon EM, et al. Factor XII-induced mitogenesis is mediated via a distinct signal transduction pathway that activates a mitogen-activated protein kinase. Proc. Natl. Acad. Sci. USA 93: 2174-2179, 1996. PubMed: 8700904
32530: Zhang X, Smith CD. Microtubule effects of welwistatin, a cyanobacterial indolinone that circumvents multiple drug resistance. Mol. Pharmacol. 49: 288-294, 1996. PubMed: 8632761
ATCC Number：CRL-1476?
數(shù)量：大量
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：DB1X
組織來源：medial layer
是否是腫瘤細(xì)胞：0
物種來源：大鼠
運(yùn)輸方式：凍存運(yùn)輸
器官來源：其他
細(xì)胞形態(tài)：成肌細(xì)胞
規(guī)格：0.2ml Designations: A-10
Depositors: ?W Carlisle
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:myoblast
Source: Organ: aorta, thoracic
Strain: DB1X
Tissue: medial layer
Cellular Products:myokinase; creatine phosphokinase; myosin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Age: embryo
Comments:The clonal cell line A10 was derived by B. Kimes and B. Brandt from the thoracic aorta of DB1X embryonic rat and possesses many of the properties characteristic of smooth muscle cells.
The cells produce spontaneous action potentials at the stationary phase of the growth cycle and exhibit an increase in activity of the enzymes myokinase and creatine phosphokinase.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 29 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1061: Kimes BW, Brandt BL. Characterization of two putative smooth muscle cell lines from rat thoracic aorta. Exp. Cell Res. 98: 349-366, 1976. PubMed: 943301
32281: Zhang X, et al. Microfilament depletion and circumvention of multiple drug resistance by sphinxolides. Cancer Res. 57: 3751-3758, 1997. PubMed: 9288783
32468: Gordon EM, et al. Factor XII-induced mitogenesis is mediated via a distinct signal transduction pathway that activates a mitogen-activated protein kinase. Proc. Natl. Acad. Sci. USA 93: 2174-2179, 1996. PubMed: 8700904
32530: Zhang X, Smith CD. Microtubule effects of welwistatin, a cyanobacterial indolinone that circumvents multiple drug resistance. Mol. Pharmacol. 49: 288-294, 1996. PubMed: 8632761
