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細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：小鼠
器官來源：脾
細胞形態(tài)：淋巴樣
品系：BALB/c
ATCC Number：CRL-1841?
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
數(shù)量：大量
規(guī)格：0.1ml Designations: HT-2 clone A5E
Depositors: ?JF Weaver, A Creasey
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast


Source: Organ: spleen
Strain: BALB/c
Cell Type: IL-2 dependent T lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:interleukin 2 (IL-2)
Antigen Expression:Thy-1
Comments:The HT-2 cell line was derived in 1979 by J. Watson at the University of Auckland from a culture of BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2.
The A5E clone was selected to provide a homogeneous population that is highly sensitive to IL-2.
The line is dependent on IL-2 for growth and can be used to measure IL-2 activity in a very rapid and sensitive assay.
The cells are commonly used for characterization of lymphokines and cytokines.
In addition to IL-2, substantial proliferation is seen in the presence of interleukin 4 (interleukin-4, IL-4, also known as BSF-1).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100-200 IU/ml IL-2, 90%; fetal bovine serum, 10%Alternatively, IL-2 in the form of Rat T-STIM factor with Con A (available from Becton Dickinson, Cat.# 354115) may be used at 10% by volume.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Subcuture every two days at 3.5 X 10 exp4 viable cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 1763: Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804
1764: Ho SN, et al. Differential bioassay of interleukin-2 and interleukin-4. J. Immunol. Methods 98: 99-104, 1987. PubMed: 3494085
4396: Lee F, et al. Isolation and characterization of a mouse interleukin cDNa clone that expresses B-cell stimulatory factor 1 activities and T-cell and mast-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 2061-2065, 1986. PubMed: 3083412
22359: Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524
22459: . . J. Biol. Response Modif. 4: 96-109, 1985.
23462: Kupper T, et al. Autocrine growth of T cells independent of interleukin 2: identification of interleukin 4 (IL 4, BSF-1) as an autocrine growth factor for a cloned antigen-specific helper T cell. J. Immunol. 138: 4280-4287, 1987. PubMed: 2953803
23463: Woods A, et al. Granulocyte-macrophage colony stimulating factor produced by cloned L3T4a+, class II-restricted T cells induces HT-2 cells to proliferate. J. Immunol. 138: 4293-4297, 1987. PubMed: 2953805
33122: Kotanides H, Reich NC. Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene. J. Biol. Chem. 271: 25555-25561, 1996. PubMed: 8810328