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ATCC Number：CRL-1853?
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b
生長狀態(tài)：懸浮生長
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：小鼠
規(guī)格：0.5mg Designations: 2H6-C2
Depositors: ?CA Whetstone
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against Bovine herpesvirus 1 (BHV-1, also known as infectious bovine rhinotracheitis virus (IBRV))
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with BHV-1.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the gD glycoprotein of BHV-1 and is used by the National Veterinary Services Laboratories of the USDA for in vitro potency testing of adjuvantated, inactivated BHV-1 vaccines.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 24382: . . Conf. Res. Workers Anim. Dis. : 54, 1987.