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平臺編號：bio-68360
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國際編號：CRL-1844?
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細胞信息： 9-A5
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
9-A5
數(shù)量：大量
ATCC Number：CRL-1844?
是否是腫瘤細胞：0
物種來源：小鼠
細胞形態(tài)：淋巴樣
免疫類型：IgG1
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
規(guī)格：48T Designations: 9-A5
Depositors: ?HL Leffert
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against rat Na,K dependent ATPase
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with purified rat Na,K dependent ATPase.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody binds to and inhibits the catalytic activity of rat Na,K dependent ATPase.
The antibody appears to bind to the alpha subunit of the enzyme.
The antibody has been used to affinity purify the enzyme, and in immunohistocytochemical localization of the enzyme in rat tissues.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 1626: Schenk DB, et al. Use of a monoclonal antibody to quantify (Na+, K+) ATPase activity and sites in normal and regenerating rat liver. J. Biol. Chem. 259: 14941-14951, 1984. PubMed: 6094583
1627: Sutherland E, et al. Biochemical localization of hepatic surface-membrane Na+, K+-ATPase activity depends on membrane lipid fluidity. Proc. Natl. Acad. Sci. USA 85: 8673-8677, 1988. PubMed: 2847169
1628: Schenk DB, Leffert HL. Monoclonal antibodies to rat Na+, K+-ATPase block enzymatic activity. Proc. Natl. Acad. Sci. USA 80: 5281-5285, 1983. PubMed: 6310568
1629: Leffert HL, et al. Hepatic (Na+, K+)-ATPase: a current view of its structure, function and localization in rat liver as revealed by studies with monoclonal antibodies. Hepatology 5: 501-507, 1985. PubMed: 2987099
1630: Hubert JJ, et al. Rat hepatic (Na+, K+)-ATPase: alpha-Subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping. Biochemistry 25: 4156-4163, 1986. PubMed: 3017414
ATCC Number：CRL-1844?
是否是腫瘤細胞：0
物種來源：小鼠
細胞形態(tài)：淋巴樣
免疫類型：IgG1
生長狀態(tài)：懸浮生長
運輸方式：凍存運輸
規(guī)格：48T Designations: 9-A5
Depositors: ?HL Leffert
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against rat Na,K dependent ATPase
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with purified rat Na,K dependent ATPase.
Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.
The antibody binds to and inhibits the catalytic activity of rat Na,K dependent ATPase.
The antibody appears to bind to the alpha subunit of the enzyme.
The antibody has been used to affinity purify the enzyme, and in immunohistocytochemical localization of the enzyme in rat tissues.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 1626: Schenk DB, et al. Use of a monoclonal antibody to quantify (Na+, K+) ATPase activity and sites in normal and regenerating rat liver. J. Biol. Chem. 259: 14941-14951, 1984. PubMed: 6094583
1627: Sutherland E, et al. Biochemical localization of hepatic surface-membrane Na+, K+-ATPase activity depends on membrane lipid fluidity. Proc. Natl. Acad. Sci. USA 85: 8673-8677, 1988. PubMed: 2847169
1628: Schenk DB, Leffert HL. Monoclonal antibodies to rat Na+, K+-ATPase block enzymatic activity. Proc. Natl. Acad. Sci. USA 80: 5281-5285, 1983. PubMed: 6310568
1629: Leffert HL, et al. Hepatic (Na+, K+)-ATPase: a current view of its structure, function and localization in rat liver as revealed by studies with monoclonal antibodies. Hepatology 5: 501-507, 1985. PubMed: 2987099
1630: Hubert JJ, et al. Rat hepatic (Na+, K+)-ATPase: alpha-Subunit isolation by immunoaffinity chromatography and structural analysis by peptide mapping. Biochemistry 25: 4156-4163, 1986. PubMed: 3017414
