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細胞類型：其他細胞類型
生長狀態(tài)：貼壁生長
是否是腫瘤細胞：0
物種來源：人
數(shù)量：大量
器官來源：大腦
年限：fetus, first trimester
ATCC Number：CRL-8621?
細胞形態(tài)：成纖維樣
運輸方式：凍存運輸
規(guī)格：48T Designations: SVG p12
Depositors: ?The United States of America
Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast


Source: Organ: brain
Cell Type: astroglia; SV40 transformed
Cellular Products:SV40 T protein; glial fibrillary acidic protein (GFAP)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: fetus, first trimester
Comments:The SVG p12 cell line was established by transfecting cultured human fetal glial cells from brain material dissected from 8 to 12 week old embryos with DNA from an ori - mutant of SV40.
The cells express SV40 T antigen.
The are susceptible to infection by JC virus, and may be useful in, detecting and cultivating other human neurotropic viruses.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels. Seed new flasks (75 sq. cm.) with 5 X 10(5) cells.
6. Incubate cultures at 37?C.
   
   Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nirogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 21915: Major EO. Immortal line of human fetal glial cells. US Patent 4,707,448 dated Nov 17 1987