-
平臺編號：bio-68368
-
國際編號：CCL-106?
-
細胞信息： LLC-RK1
-
規(guī)格：frozen
-
用途：ATCC原裝細胞
-
服務(wù)費用：
加載中…… - 訂購
-
注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
LLC-RK1
生長狀態(tài)：貼壁生長
是否是腫瘤細胞：0
物種來源：其他
細胞形態(tài)：上皮樣
ATCC Number：CCL-106?
相關(guān)疾病：正常
器官來源：腎臟
數(shù)量：大量
運輸方式：凍存運輸
規(guī)格：0.5mg Designations: LLC-RK1
Depositors: ?RN Hull
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Oryctolagus cuniculus
Morphology:epithelial
Source: Organ: kidney
Disease: normal
Cellular Products:plasminogen activator; keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 1
Comments:The cells are positive for keratin by immunoperoxidase staining.
The cells are negative for bovine viral diarrhea virus (BVDV). [22883 ]
Propagation: ATCC complete growth medium: Medium 199 with 1.12 g/L sodium bicarbonate, 90%; horse serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Please note, this cell line is extremely sensitive to trypsin.Remove the spent medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Transfer the cell suspension to a centrifuge tube, and spin at approximately 125 x g for 5 to 10 minutes. Remove the supernatant, add fresh medium to the pellet, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
References: 22354: Hull RN, et al. Development and characteristics of the rabbit kidney cell line strain, LLC-RK. Proc. Soc. Exp. Biol. Med. 118: 1054-1059, 1965. PubMed: 14277663
22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
26280: Hull RN, Butorac G. The utility of rabbit kidney cell strain, LLC-RK to rubella virus studies. Am. J. Epidemiol. 83: 509-517, 1966. PubMed: 4956596
是否是腫瘤細胞：0
物種來源：其他
細胞形態(tài)：上皮樣
ATCC Number：CCL-106?
相關(guān)疾病：正常
器官來源：腎臟
數(shù)量：大量
運輸方式：凍存運輸
規(guī)格：0.5mg Designations: LLC-RK1
Depositors: ?RN Hull
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Oryctolagus cuniculus
Morphology:epithelial
Source: Organ: kidney
Disease: normal
Cellular Products:plasminogen activator; keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance:poliovirus 1
Comments:The cells are positive for keratin by immunoperoxidase staining.
The cells are negative for bovine viral diarrhea virus (BVDV). [22883 ]
Propagation: ATCC complete growth medium: Medium 199 with 1.12 g/L sodium bicarbonate, 90%; horse serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Please note, this cell line is extremely sensitive to trypsin.Remove the spent medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Transfer the cell suspension to a centrifuge tube, and spin at approximately 125 x g for 5 to 10 minutes. Remove the supernatant, add fresh medium to the pellet, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
References: 22354: Hull RN, et al. Development and characteristics of the rabbit kidney cell line strain, LLC-RK. Proc. Soc. Exp. Biol. Med. 118: 1054-1059, 1965. PubMed: 14277663
22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
26280: Hull RN, Butorac G. The utility of rabbit kidney cell strain, LLC-RK to rubella virus studies. Am. J. Epidemiol. 83: 509-517, 1966. PubMed: 4956596
