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細(xì)胞形態(tài)：成纖維樣
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
ATCC Number：CRL-1888?
品系：NIH/Swiss
器官來(lái)源：胚胎
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
年限：embryo
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
規(guī)格：0.5ml Designations: EJ-6-2-Bam-6a
Depositors: ?RA Weinberg
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast


Source: Organ: embryo
Strain: NIH/Swiss
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Age: embryo
Comments:This line was established by C. Shih et al. in 1981 by transfecting NIH/3T3 cells with Bam H1 digested DNA from EJ-6-2 cells (NIH/3T3 cells transformed with DNA from the human EJ bladder carcinoma EJ).
The cells are morphologically transformed and highly tumorigenic in BALB/c and NSF mice.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; bovine calf serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA, rinse and remove trypsin and let the culture sit at 37C until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture twice weekly.
References: 22917: Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109
26163: Shih C, et al. Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature 290: 261-264, 1981. PubMed: 7207618