-
平臺編號：bio-68390
-
國際編號：CRL-10781?
-
細胞信息： MCF-10-2A
-
規(guī)格：frozen
-
用途：ATCC原裝細胞
-
服務(wù)費用：
加載中…… - 訂購
-
注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
MCF-10-2A
細胞形態(tài)：上皮樣
ATCC Number：CRL-10781?
運輸方式：凍存運輸
相關(guān)疾?。浩渌膊?br /> 是否是腫瘤細胞：0
物種來源：人
數(shù)量：大量
年限：36 years
器官來源：乳房
生長狀態(tài)：貼壁生長
規(guī)格：0.1ml Designations: MCF-10-2A
Depositors: ?Michigan Cancer Foundation
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: mammary gland; breast
Disease: fibrocystic disease
Cellular Products:cytokeratin 19
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Receptors:epidermal growth factor (EGF); insulin; glucocorticoid
Tumorigenic:No
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,17
Cytogenetic Analysis:modal number = 40 to 43; XX; five marker chromosomes; double minutes
Age: 36 years
Gender: female
Comments:The MCF-10-2A cell line is a non-tumorigenic epithelial cell line that does not undergo senescence after exposure to high calcium levels.
The line was produced by long term culture in serum free medium with low Ca++ concentration.
MCA-10-2A was derived from adherent cells in the population.
Cells derived from a floating population are available (see MCF-10-2F, ATCC CRL-10780 ).
The cells are positive for epithelial cytokeratins and milk fat globule antigen.
They exhibit three dimensional growth in collagen, and infrequently form domes in confluent cultures.
This line was established from the same tumor material as MCF 10A (ATCC CRL-10317 ) and MCF 10F (ATCC CRL-10318 ).
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium supplemented with 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.01 mg/ml insulin and 500 ng/ml hydrocortisone, 95%; horse serum, 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:derived from same individual:ATCC CRL-10317
derived from same individual:ATCC CRL-10318
derived from same individual:ATCC CRL-10780
References: 21968: Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991
22025: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993
22043: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,238,840 dated Aug 24 1993
22248: Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007
23084: Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513
23085: Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506
ATCC Number：CRL-10781?
運輸方式：凍存運輸
相關(guān)疾?。浩渌膊?br /> 是否是腫瘤細胞：0
物種來源：人
數(shù)量：大量
年限：36 years
器官來源：乳房
生長狀態(tài)：貼壁生長
規(guī)格：0.1ml Designations: MCF-10-2A
Depositors: ?Michigan Cancer Foundation
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: mammary gland; breast
Disease: fibrocystic disease
Cellular Products:cytokeratin 19
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host
Receptors:epidermal growth factor (EGF); insulin; glucocorticoid
Tumorigenic:No
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 11,12
D5S818: 10,13
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,17
Cytogenetic Analysis:modal number = 40 to 43; XX; five marker chromosomes; double minutes
Age: 36 years
Gender: female
Comments:The MCF-10-2A cell line is a non-tumorigenic epithelial cell line that does not undergo senescence after exposure to high calcium levels.
The line was produced by long term culture in serum free medium with low Ca++ concentration.
MCA-10-2A was derived from adherent cells in the population.
Cells derived from a floating population are available (see MCF-10-2F, ATCC CRL-10780 ).
The cells are positive for epithelial cytokeratins and milk fat globule antigen.
They exhibit three dimensional growth in collagen, and infrequently form domes in confluent cultures.
This line was established from the same tumor material as MCF 10A (ATCC CRL-10317 ) and MCF 10F (ATCC CRL-10318 ).
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium supplemented with 20 ng/ml epidermal growth factor, 100 ng/ml cholera toxin, 0.01 mg/ml insulin and 500 ng/ml hydrocortisone, 95%; horse serum, 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:derived from same individual:ATCC CRL-10317
derived from same individual:ATCC CRL-10318
derived from same individual:ATCC CRL-10780
References: 21968: Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991
22025: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993
22043: Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,238,840 dated Aug 24 1993
22248: Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007
23084: Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513
23085: Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506
