細胞類型:B淋巴細胞
生長狀態(tài):懸浮生長
運輸方式:凍存運輸
細胞形態(tài):淋巴樣
是否是腫瘤細胞:0
物種來源:小鼠
免疫類型:IgA; lambda 2 light chain
品系:BALB/c
數(shù)量:大量
ATCC Number:TIB-23?
相關疾?。憾喟l(fā)性骨髓瘤
規(guī)格:0.2ml Designations: MOPC 315
Depositors: ?M Cohn
Isotype: IgA; lambda 2 light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus
Morphology:lymphoblast
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against DNP and TNP substituted proteins
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Antigen Expression:H-2d
Comments:This line was established from the MOPC 315 plasmacytoma (induced with mineral oil in a BALB/c mouse).
The cells are killed by 10 nM dexamethasone.
Initial recovery from the frozen state is slow, and approximately 7 to 10 days are required before any noticeable cell growth occurs.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1114: Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956
22228: Halpern MS, Coffman RL. Polymer formation and J chain synthesis in mouse plasmacytomas. J. Immunol. 109: 674-680, 1972. PubMed: 5074326
23163: Potter M. Immunoglobulin-producing tumors and myeloma proteins of mice. Physiol. Rev. 52: 631-719, 1972. PubMed: 4555516