![DG-75 [D.G.-75]](http://www.family998.com/static/images/cells-no_picture.jpg)
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平臺(tái)編號(hào)：bio-68398
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國(guó)際編號(hào)：CRL-2625?
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細(xì)胞信息： DG-75 [D.G.-75]
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
DG-75 [D.G.-75]
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
年限：10 years
細(xì)胞形態(tài)：淋巴樣
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
ATCC Number：CRL-2625?
相關(guān)疾?。毫馨土?br /> 運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.3ml Designations: DG-75 [D.G.-75]
Depositors: ?H Ben-Bassat
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: Burkitt's lymphoma
Derived from metastatic site: pleural effusion
Cell Type: B lymphocyte;
Cellular Products:surface immunoglobulin (IgM, k) [53289 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1975
Applications:The cells are reported to be negative for Epstein-Barr virus and E-rosette negative [PubmedID: 188769].
Receptors:Fc, not expressed [53282 ]
complement (C3), not expressed [53282 ]
Antigen Expression:CD10; Homo sapiens, expressed [53289 ]
CD19; Homo sapiens, expressed (weakly expressed) ( [53289 ] weakly expressed)
CD20; Homo sapiens [53289 ]
CD23; Homo sapiens [53289 ]
CD38; Homo sapiens, expressed [53289 ]
CD39; Homo sapiens [53289 ]
Cytogenetic Analysis:t(8:14)(q24;q32) [53289 ]
Age: 10 years
Gender: male
Ethnicity: White
Comments:The cells are reported to be negative for Epstein-Barr virus and E-rosette negative [PubmedID: 188769].
A culture submitted to the ATCC in July of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. After a six-week period following treatment, the cells were assayed for mycoplasma, by Hoechst stain, PCR and by the standard culture test. All tests were negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 3 X 10(5) and 3 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 20 to 24 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
References: 53282: Ben-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769
53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
數(shù)量：大量
年限：10 years
細(xì)胞形態(tài)：淋巴樣
細(xì)胞類型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
ATCC Number：CRL-2625?
相關(guān)疾?。毫馨土?br /> 運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.3ml Designations: DG-75 [D.G.-75]
Depositors: ?H Ben-Bassat
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Disease: Burkitt's lymphoma
Derived from metastatic site: pleural effusion
Cell Type: B lymphocyte;
Cellular Products:surface immunoglobulin (IgM, k) [53289 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1975
Applications:The cells are reported to be negative for Epstein-Barr virus and E-rosette negative [PubmedID: 188769].
Receptors:Fc, not expressed [53282 ]
complement (C3), not expressed [53282 ]
Antigen Expression:CD10; Homo sapiens, expressed [53289 ]
CD19; Homo sapiens, expressed (weakly expressed) ( [53289 ] weakly expressed)
CD20; Homo sapiens [53289 ]
CD23; Homo sapiens [53289 ]
CD38; Homo sapiens, expressed [53289 ]
CD39; Homo sapiens [53289 ]
Cytogenetic Analysis:t(8:14)(q24;q32) [53289 ]
Age: 10 years
Gender: male
Ethnicity: White
Comments:The cells are reported to be negative for Epstein-Barr virus and E-rosette negative [PubmedID: 188769].
A culture submitted to the ATCC in July of 2001 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline. After a six-week period following treatment, the cells were assayed for mycoplasma, by Hoechst stain, PCR and by the standard culture test. All tests were negative.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 3 X 10(5) and 3 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 20 to 24 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
Cell culture tested DMSO:ATCC 4-X
References: 53282: Ben-Bassat H, et al. Establishment in continuous culture of a new type of lymphocyte from a "Burkitt like" malignant lymphoma (line D.G.-75). Int. J. Cancer 19: 27-33, 1977. PubMed: 188769
53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
