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平臺編號：bio-68400
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國際編號：CRL-2217?
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細胞信息： BpRc1
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
BpRc1
細胞形態(tài)：上皮樣
運輸方式：凍存運輸
品系：C57L
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
器官來源：肝
生長狀態(tài)：貼壁生長
ATCC Number：CRL-2217?
相關疾?。焊伟?br /> 規(guī)格：0.1ml Designations: BpRc1
Depositors: ?J Whitlock
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:epithelial
Source: Strain: C57L
Organ: liver
Disease: hepatoma
Cellular Products:cytochrome P450IA1
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:aryl hydrocarbon (Ah)
Comments:BpRc1 is a Class II variant of the Hepa-1clc7 (see ATCC CRL-2026 ) hepatoma cell line which was derived from the Hepa-1 cell line established by Bernhard, et al. This cell line along with the wild-type Hepa-1clc7 and the class I variant tao BpRcl (see ATCC CRL-2218 ) can be used to study dioxin (TCDD) induction of the CYPIA1 gene, which precedes increased levels of p450IA1 enzyme. The p450IAI enzyme catalyzes hydroxylase activity which results in the oxygenation of aromatic substrates such as the environmental carcinogen benzo[a]pyrene. Induction of transcription requires the aryl hydrocarbon hydroxylase (Ah) receptor to bind TCDD and transport of the TCDD receptor complex into the nucleus where interaction with a dioxin responsive enhancer (DRE) upstream of the CYPIA1 gene facilitates increased transcription.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 12 to 24 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22320: Bernhard HP, et al. Expression of liver phenotypes in cultured mouse hepatoma cells: synthesis and secretion of serum albumin. Dev. Biol. 35: 83-96, 1973. PubMed: 4362668
運輸方式：凍存運輸
品系：C57L
是否是腫瘤細胞：0
物種來源：小鼠
數(shù)量：大量
器官來源：肝
生長狀態(tài)：貼壁生長
ATCC Number：CRL-2217?
相關疾?。焊伟?br /> 規(guī)格：0.1ml Designations: BpRc1
Depositors: ?J Whitlock
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:epithelial
Source: Strain: C57L
Organ: liver
Disease: hepatoma
Cellular Products:cytochrome P450IA1
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:aryl hydrocarbon (Ah)
Comments:BpRc1 is a Class II variant of the Hepa-1clc7 (see ATCC CRL-2026 ) hepatoma cell line which was derived from the Hepa-1 cell line established by Bernhard, et al. This cell line along with the wild-type Hepa-1clc7 and the class I variant tao BpRcl (see ATCC CRL-2218 ) can be used to study dioxin (TCDD) induction of the CYPIA1 gene, which precedes increased levels of p450IA1 enzyme. The p450IAI enzyme catalyzes hydroxylase activity which results in the oxygenation of aromatic substrates such as the environmental carcinogen benzo[a]pyrene. Induction of transcription requires the aryl hydrocarbon hydroxylase (Ah) receptor to bind TCDD and transport of the TCDD receptor complex into the nucleus where interaction with a dioxin responsive enhancer (DRE) upstream of the CYPIA1 gene facilitates increased transcription.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and remove trypsin, Add an additional 1 to 2 ml of trypsin solution, and allow the flask to sit at room temperature (or 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 12 to 24 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22320: Bernhard HP, et al. Expression of liver phenotypes in cultured mouse hepatoma cells: synthesis and secretion of serum albumin. Dev. Biol. 35: 83-96, 1973. PubMed: 4362668
