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運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
ATCC Number：TIB-191?
是否是腫瘤細(xì)胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
規(guī)格：0.3ml Designations: 1B7.11
Depositors: ?H Nariuchi
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with TNP-KLH.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with TNP and forms rosettes with TNP conjugated sheep red blood cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%