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平臺編號：bio-68416
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國際編號：CCL-110?
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細(xì)胞信息： Detroit 551
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Detroit 551
ATCC Number：CCL-110?
相關(guān)疾?。赫?br /> 細(xì)胞形態(tài)：成纖維樣
運(yùn)輸方式：凍存運(yùn)輸
器官來源：皮膚
細(xì)胞類型：成纖維細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
生長狀態(tài)：貼壁生長
年限：fetus
數(shù)量：大量
規(guī)格：0.2ml Designations: Detroit 551
Depositors: ?CS Stulberg
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1965
Applications:Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
Virus Susceptibility:SV40 virus
Human poliovirus 1
Herpes simplex virus
Vesicular stomatitis virus
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 18
Cytogenetic Analysis:normal human female; diploid
Isoenzymes: G6PD, B
Age: fetus
Gender: female
Ethnicity: Caucasian
Comments:The cells have a finite lifespan of about 25 serial passages from the tissue of origin.
They can be transformed by SV40.
Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
相關(guān)疾?。赫?br /> 細(xì)胞形態(tài)：成纖維樣
運(yùn)輸方式：凍存運(yùn)輸
器官來源：皮膚
細(xì)胞類型：成纖維細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
生長狀態(tài)：貼壁生長
年限：fetus
數(shù)量：大量
規(guī)格：0.2ml Designations: Detroit 551
Depositors: ?CS Stulberg
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1965
Applications:Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
Virus Susceptibility:SV40 virus
Human poliovirus 1
Herpes simplex virus
Vesicular stomatitis virus
DNA Profile (STR):Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 18
Cytogenetic Analysis:normal human female; diploid
Isoenzymes: G6PD, B
Age: fetus
Gender: female
Ethnicity: Caucasian
Comments:The cells have a finite lifespan of about 25 serial passages from the tissue of origin.
They can be transformed by SV40.
Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 21405: Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
