細(xì)胞形態(tài):成纖維樣
數(shù)量:大量
ATCC Number:CCL-197?
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品系:C57BL/10
組織來(lái)源:skeletal muscle
細(xì)胞類型:成纖維細(xì)胞
運(yùn)輸方式:凍存運(yùn)輸
是否是腫瘤細(xì)胞:0
物種來(lái)源:小鼠
生長(zhǎng)狀態(tài):貼壁生長(zhǎng)
年限:30 days
規(guī)格:0.1ml Designations: NOR-10
Depositors: ?BC Starcher
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Tissue: skeletal muscle
Strain: C57BL/10
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:H-2b
Age: 30 days
Comments:This may be used as a matched control for ATCC CCL-198 (BLO-1).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.1% trypsin for about 10 minutes, dislodge cells and centrifuge to remove from trypsin. Add fresh medium to cell pellet, resuspend cells in fresh medium and dispense into new flasks. Subculture every 3 to 5 days.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%