![8E5 [derivative of CEM]](http://www.family998.com/static/images/cells-no_picture.jpg)
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平臺(tái)編號(hào)：bio-68431
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國際編號(hào)：CRL-8993?
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細(xì)胞信息： 8E5 [derivative of CEM]
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
8E5 [derivative of CEM]
生長狀態(tài)：懸浮生長
細(xì)胞形態(tài)：淋巴樣
細(xì)胞類型：其他細(xì)胞類型
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：人
數(shù)量：大量
ATCC Number：CRL-8993?
相關(guān)疾?。喊籽?br /> 年限：4 years
器官來源：外周血
規(guī)格：48T Designations: 8E5 [derivative of CEM]
Depositors: ?The United States of America
Biosafety Level:2 [Cells Contain RETROVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: lymphoblasthuman immunodeficiency virus (HIV) positive
Cellular Products:most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:transferrin
Antigen Expression:CD4 +; CD5 +
Age: 4 years
Gender: female
Ethnicity: Caucasian
Comments:The cells contain a single defective proviral genome of HTLV III (HIV, LAV).
All of the major structural protein are constitutively expressed with the exception of the p64 and p34 proteins.
No infectious virus is produced; however, co-cultivation with Leu-3 + cells leads to syncytia formation.
The cells have been cured of a mycoplasma contamination.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CCL-119
References: 3870: Powell DM, et al. Cell line producing AIDS viral antigens without producing infectious virus particles. US Patent 4,752,565 dated Jun 21 1988
細(xì)胞形態(tài)：淋巴樣
細(xì)胞類型：其他細(xì)胞類型
運(yùn)輸方式：凍存運(yùn)輸
是否是腫瘤細(xì)胞：0
物種來源：人
數(shù)量：大量
ATCC Number：CRL-8993?
相關(guān)疾?。喊籽?br /> 年限：4 years
器官來源：外周血
規(guī)格：48T Designations: 8E5 [derivative of CEM]
Depositors: ?The United States of America
Biosafety Level:2 [Cells Contain RETROVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: lymphoblasthuman immunodeficiency virus (HIV) positive
Cellular Products:most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:transferrin
Antigen Expression:CD4 +; CD5 +
Age: 4 years
Gender: female
Ethnicity: Caucasian
Comments:The cells contain a single defective proviral genome of HTLV III (HIV, LAV).
All of the major structural protein are constitutively expressed with the exception of the p64 and p34 proteins.
No infectious virus is produced; however, co-cultivation with Leu-3 + cells leads to syncytia formation.
The cells have been cured of a mycoplasma contamination.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CCL-119
References: 3870: Powell DM, et al. Cell line producing AIDS viral antigens without producing infectious virus particles. US Patent 4,752,565 dated Jun 21 1988
