ATCC Number:HB-8051?
運(yùn)輸方式:凍存運(yùn)輸
是否是腫瘤細(xì)胞:0
物種來(lái)源:小鼠
細(xì)胞形態(tài):淋巴樣
免疫類型:IgM; kappa light chain
生長(zhǎng)狀態(tài):懸浮生長(zhǎng)
數(shù)量:大量
規(guī)格:48T Designations: F5-A-1/22.8.13
Depositors: ?Research Corporation
Isotype: IgM; kappa light chain
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human prostate antigen (PA)
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Comments:Animals were immunized with purified PA.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: fetal bovine serum, 90%; DMSO, 10%
References: 3874: Wang MC, et al. Purified human prostate antigen. US Patent 4,446,122 dated May 1 1984
58335: Wang MC, et al. Purification of a human prostate specific antigen. Invest. Urol. 17: 159-163, 1979. PubMed: 89106