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平臺編號：bio-68441
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國際編號：CRL-2622?
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細(xì)胞信息： CCD-1132Sk
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
CCD-1132Sk
ATCC Number：CRL-2622?
相關(guān)疾?。赫?br /> 運(yùn)輸方式：凍存運(yùn)輸
年限：18 years
器官來源：皮膚
細(xì)胞形態(tài)：成纖維樣
數(shù)量：大量
細(xì)胞類型：成纖維細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
規(guī)格：0.1ml Designations: CCD-1132Sk
Depositors: ?F.Haq, ATCC
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Organism: Homo sapiens deposited as human
Morphology:fibroblast
Source: Organ: skin
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 18 years
Gender: female
Ethnicity: Black
Comments:The cell line was established from skin taken from the breast of a female with macromastia.
Cells senesce after approximately 56 population doublings.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
相關(guān)疾?。赫?br /> 運(yùn)輸方式：凍存運(yùn)輸
年限：18 years
器官來源：皮膚
細(xì)胞形態(tài)：成纖維樣
數(shù)量：大量
細(xì)胞類型：成纖維細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：人
規(guī)格：0.1ml Designations: CCD-1132Sk
Depositors: ?F.Haq, ATCC
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Organism: Homo sapiens deposited as human
Morphology:fibroblast
Source: Organ: skin
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 18 years
Gender: female
Ethnicity: Black
Comments:The cell line was established from skin taken from the breast of a female with macromastia.
Cells senesce after approximately 56 population doublings.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
