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EIAV 12E8.1
    EIAV 12E8.1
  • 平臺編號:bio-68445
  • 國際編號:HB-8917?
  • 細胞信息: EIAV 12E8.1
  • 規(guī)格:frozen
  • 用途:ATCC原裝細胞
  • 服務(wù)費用:
    加載中……
  • 訂購
  • 注意事項:僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準)

數(shù)量:大量
細胞形態(tài):淋巴樣
免疫類型:IgG1
ATCC Number:HB-8917?
是否是腫瘤細胞:0
物種來源:小鼠
生長狀態(tài):懸浮生長
運輸方式:凍存運輸
規(guī)格:0.5ml Designations: EIAV 12E8.1
Depositors: ?Fermenta Animal Health Company
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against equine infectious anemia virus (EIA, EIAV) core antigen (p26)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with p26 prepared from the Wyoming strain of EIA virus.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 85%; fetal bovine serum, 15%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: DMEM, 70%; fetal bovine serum, 20%; DMSO, 10%
References: 3914: Matsushita T, et al. Method for the detection of equine infectious anemia and other retrovirus infections using a competitive enzyme-linked immunoabsorbent assay and reagents useful in the same. US Patent 4,806,467 dated Feb 21 1989

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