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平臺(tái)編號(hào)：bio-68450
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國(guó)際編號(hào)：CRL-1887?
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細(xì)胞信息： B104-1-1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
B104-1-1
年限：embryo
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：NIH/Swiss
ATCC Number：CRL-1887?
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：胚胎
數(shù)量：大量
細(xì)胞形態(tài)：成纖維樣
規(guī)格：0.1ml Designations: B104-1-1
Depositors: ?RA Weinberg
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo
Comments:This line was established by A.L. Schechter et al. in 1984 by transfecting NIH/3T3 cells with EcoR1 digested DNA from the rat neuroblastoma cell line B-104.
The cells contain the neu transforming gene which codes for a 185000 dalton antigen designated p185.
The p185 protein is strongly associated with the presence of glioblastoma and neuroblastoma oncogenes.
The neu oncogene is homologous to the erb-B oncogene, and p185 is serologically similar to the epidermal growth factor receptor.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; bovine calf serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA, rinse and remove trypsin and let the culture sit at 37C until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture twice weekly.
References: 22917: Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
品系：NIH/Swiss
ATCC Number：CRL-1887?
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：胚胎
數(shù)量：大量
細(xì)胞形態(tài)：成纖維樣
規(guī)格：0.1ml Designations: B104-1-1
Depositors: ?RA Weinberg
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus
Morphology:fibroblast
Source: Organ: embryo
Strain: NIH/Swiss
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo
Comments:This line was established by A.L. Schechter et al. in 1984 by transfecting NIH/3T3 cells with EcoR1 digested DNA from the rat neuroblastoma cell line B-104.
The cells contain the neu transforming gene which codes for a 185000 dalton antigen designated p185.
The p185 protein is strongly associated with the presence of glioblastoma and neuroblastoma oncogenes.
The neu oncogene is homologous to the erb-B oncogene, and p185 is serologically similar to the epidermal growth factor receptor.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; bovine calf serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA, rinse and remove trypsin and let the culture sit at 37C until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture twice weekly.
References: 22917: Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109
