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年限：adult; day 14 of estrous cycle
運(yùn)輸方式：凍存運(yùn)輸
組織來源：endometrium
生長狀態(tài)：貼壁生長
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：牛
器官來源：子宮
ATCC Number：CRL-2398?
相關(guān)疾?。赫?br /> 細(xì)胞形態(tài)：上皮樣
規(guī)格：0.1ml Designations: BEND
Depositors: ?TR Hansen, KJ Austin, GA Johnson
Biosafety Level:2 [Cells contain bovine viral diarrhea virus (BVDV) ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Bos taurus
Morphology:epithelial


Source: Organ: uterus
Tissue: endometrium
Disease: normal
Cellular Products:ubiquitin cross-reactive protein (UCRP) and alpha chemokines in response to interferon
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Laramie Wyoming, United States
Isolation date: 1997
Age: adult; day 14 of estrous cycle
Gender: female
Comments:The BEND cell line was derived from the uterine endometrium of a normal female cow on day 14 of the estrous cycle in 1997.
The cells produce the pregnancy associated protein called bovine ubiquitin cross-reactive protein (UCRP) and alpha chemokines in response to the cytokine interferon-tau. [38882 ]
BEND was submitted to the American Type Culture Collection in January, 1998 at passage 6.
Tests for bovine viral diarrhea virus (BVDV) at the ATCC have indicated that BEND cells are positive for BVDV by immunofluorescence.
Propagation: ATCC complete growth medium: 1:1 mixture of Ham's F12 and Eagle's Minimal Essential medium with Earle's BSS (D-valine modification) with 1.5 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.034 g/L D-valine, 10% heat-inactivated fetal bovine serum and 10% heat-inactivated horse serum
Temperature: 37.0°C
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 38881: Staggs KL, et al. Complex induction of bovine uterine proteins by interferon-tau. Biol. Reprod. 59: 293-297, 1998. PubMed: 9687298
38882: Hansen TR, et al. Transient ubiquitin cross-reactive protein gene expression in the bovine endometrium. Endocrinology 138: 5079-5082, 1997. PubMed: 9348245
45046: Austin KJ, et al. Pregnancy-specific protein B induces release of an alpha chemokine in bovine endometrium. Endocrinology 140: 542-545, 1999. PubMed: 9886868
45047: Johnson GA, et al. Endometrial ISG17 mRNA and a related mRNA are induced by interferon-tau and localized to glandular epithelial and stromal cells from pregnant cows. Endocrine 10: 243-252, 1999. PubMed: 10484288
45048: Perry DJ, et al. Cloning of interferon-stimulated gene 17: the promoter and nuclear proteins that regulate transcription. Mol. Endocrinol. 13: 1197-1206, 1999. PubMed: 10406469