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平臺(tái)編號(hào)：bio-68455
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國(guó)際編號(hào)：CCL-215?
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細(xì)胞信息： CCD-25Lu
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
CCD-25Lu
數(shù)量：大量
器官來(lái)源：肺
年限：7 years
細(xì)胞類型：成纖維細(xì)胞
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
ATCC Number：CCL-215?
相關(guān)疾病：正常
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：成纖維樣
規(guī)格：0.1ml Designations: CCD-25Lu
Depositors: ?S Dilworth
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: March, 1979
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,14
D16S539: 9,11
D5S818: 12
D7S820: 8,11
THO1: 6,7
TPOX: 11,12
vWA: 17,18
Cytogenetic Analysis:normal male; diploid; stable
Age: 7 years
Gender: male
Ethnicity: Caucasian
Comments:The line was established from the lung of a patient who died of glioma of the brain stem.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Interval: Subculture every 7 to 9 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
器官來(lái)源：肺
年限：7 years
細(xì)胞類型：成纖維細(xì)胞
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
ATCC Number：CCL-215?
相關(guān)疾病：正常
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：成纖維樣
規(guī)格：0.1ml Designations: CCD-25Lu
Depositors: ?S Dilworth
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: March, 1979
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,14
D16S539: 9,11
D5S818: 12
D7S820: 8,11
THO1: 6,7
TPOX: 11,12
vWA: 17,18
Cytogenetic Analysis:normal male; diploid; stable
Age: 7 years
Gender: male
Ethnicity: Caucasian
Comments:The line was established from the lung of a patient who died of glioma of the brain stem.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Interval: Subculture every 7 to 9 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
