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平臺編號：bio-68457
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國際編號：CRL-2076?
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細(xì)胞信息： CCD-1064Sk
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
CCD-1064Sk
年限：newborn
細(xì)胞類型：成纖維細(xì)胞
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：人
ATCC Number：CRL-2076?
相關(guān)疾?。赫?br /> 運輸方式：凍存運輸
細(xì)胞形態(tài)：成纖維樣
生長狀態(tài)：貼壁生長
器官來源：皮膚
規(guī)格：0.1ml Designations: CCD-1064Sk
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: newborn
Gender: male
Comments:The line was established from skin taken from normal foreskin.
The cells are capable of approximately 54 population doublings before the onset of senescence.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
細(xì)胞類型：成纖維細(xì)胞
數(shù)量：大量
是否是腫瘤細(xì)胞：0
物種來源：人
ATCC Number：CRL-2076?
相關(guān)疾?。赫?br /> 運輸方式：凍存運輸
細(xì)胞形態(tài)：成纖維樣
生長狀態(tài)：貼壁生長
器官來源：皮膚
規(guī)格：0.1ml Designations: CCD-1064Sk
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:fibroblast
Source: Organ: skin
Disease: normal
Cell Type: fibroblast
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: newborn
Gender: male
Comments:The line was established from skin taken from normal foreskin.
The cells are capable of approximately 54 population doublings before the onset of senescence.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 3 to 4 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) for 2 to 5 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Preservation: Culture medium, 95%; DMSO, 5%
