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平臺編號：bio-68464
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國際編號：CRL-2371?
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細胞信息： C1R-B7
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
C1R-B7
器官來源：外周血
ATCC Number：CRL-2371?
數(shù)量：大量
生長狀態(tài)：混合型生長
細胞形態(tài)：淋巴樣
運輸方式：凍存運輸
細胞類型：B淋巴細胞
是否是腫瘤細胞：0
物種來源：人
規(guī)格：0.1ml Designations: C1R-B7
Depositors: ?F Grumet
Biosafety Level:2 [Cells contain herpesvirus ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:mixed; semi-attached
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:Expresses surface HLA B7; does not secrete HLA B7
DNA Profile (STR):Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8
TPOX: 8
vWA: 17
Comments:C1R-B7 is a stable transfectant cell line established in 1988 with a normal major histocompatibility complex (MHC) HLA B7 gene. It expresses surface HLA B7 but does not secrete HLA B7. [38581 ]
C1R is a human B-cell lymphoblastoid line lacking surface HLA A and B antigens. C1R was derived from Hmy.2 B-LCL by gamma irradiation followed by selection for Class I monoclonal antibodies and complement. [23312 ]
C1R-B7 may be used as a control for C1R-sB7; in CTL experiments and for producing control supernatants.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Cultures can be maintained by transferring floating cells to additional flasks.
Attached cells may be subcultured by scraping.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2020
parental cell line:ATCC CRL-1993
derived from same cell line:ATCC CRL-2369
derived from same cell line:ATCC CRL-2371
References: 23312: Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569
38581: Grumet FC, et al. Soluble form of an HLA-B7 class I antigen specifically suppresses humoral alloimmunization. Hum. Immunol. 40: 228-234, 1994. PubMed: 7960967
38582: Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968
ATCC Number：CRL-2371?
數(shù)量：大量
生長狀態(tài)：混合型生長
細胞形態(tài)：淋巴樣
運輸方式：凍存運輸
細胞類型：B淋巴細胞
是否是腫瘤細胞：0
物種來源：人
規(guī)格：0.1ml Designations: C1R-B7
Depositors: ?F Grumet
Biosafety Level:2 [Cells contain herpesvirus ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:mixed; semi-attached
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:Expresses surface HLA B7; does not secrete HLA B7
DNA Profile (STR):Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8
TPOX: 8
vWA: 17
Comments:C1R-B7 is a stable transfectant cell line established in 1988 with a normal major histocompatibility complex (MHC) HLA B7 gene. It expresses surface HLA B7 but does not secrete HLA B7. [38581 ]
C1R is a human B-cell lymphoblastoid line lacking surface HLA A and B antigens. C1R was derived from Hmy.2 B-LCL by gamma irradiation followed by selection for Class I monoclonal antibodies and complement. [23312 ]
C1R-B7 may be used as a control for C1R-sB7; in CTL experiments and for producing control supernatants.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Cultures can be maintained by transferring floating cells to additional flasks.
Attached cells may be subcultured by scraping.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2020
parental cell line:ATCC CRL-1993
derived from same cell line:ATCC CRL-2369
derived from same cell line:ATCC CRL-2371
References: 23312: Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569
38581: Grumet FC, et al. Soluble form of an HLA-B7 class I antigen specifically suppresses humoral alloimmunization. Hum. Immunol. 40: 228-234, 1994. PubMed: 7960967
38582: Hiraki DD, et al. Bioengineered soluble HLA-B7. Genesis, characterization, and occurrence of dimerization. Hum. Immunol. 40: 235-246, 1994. PubMed: 7960968
