生長狀態(tài):懸浮生長
品系:: Sprague-Dawley
ATCC Number:CRL-1604?
數(shù)量:大量
運(yùn)輸方式:凍存運(yùn)輸
是否是腫瘤細(xì)胞:0
物種來源:褐鼠
規(guī)格:1mg Designations: N1-S1
Depositors: ?JE Becker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Rattus norvegicus
Morphology:
Source: Strain : Sprague-Dawley
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: male
Comments:The N1-S1 cell line was established from a hepatoma induced by feeding 4-dimethylaminoazobenzene to a male rat.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 (4) viable cells/ml and subculture at 1 to 1.5 X 10 (6) cells/ml ( after about 3 days). Excessive clumping of cells can often be cured by trypsinizing the cells to disperse them.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Related Products:ATCC CRL-1603, N1-S1