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平臺編號：bio-68471
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國際編號：CRL-2469?
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細胞信息： EOC 20
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
EOC 20
年限：10 days juvenile
運輸方式：凍存運輸
細胞類型：其他細胞類型
生長狀態(tài)：貼壁生長
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：CRL-2469?
數量：大量
器官來源：大腦
品系：C3H/HeJ
細胞形態(tài)：單核細胞/巨噬細胞
規(guī)格：10mg Designations: EOC 20
Depositors: ?WS Walker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:macrophage
Source: Organ: brain
Strain: C3H/HeJ
Cell Type: microglia;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:colony stimulating factor 1 (CSF-1R, CD115)
Antigen Expression:CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD45 +, Ly-6C +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR +, F4/80 +/-, CD86 (B7.2) - [39974 ]
Age: 10 days juvenile
Gender: female
Comments:This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse [PubMed: 8550814]. Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. The cell line is dependent on growth factor colony stimulating factor 1 (CSF-1). Conditioned medium is made from LADMAC cells (ATCC CRL-2420?) as a source of CSF-1.The cells exhibit phagocytic activity. These cells constitutively expressed high levels of major histocompatibility complex (MHC) class II antigens and expression was upregulated by recombinant murine interferon-gamma. The cells may be used to characterize the role of brain macrophages.C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove 75% of the media. Scrape off the attached cells and transfer into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
purified RNA:ATCC CRL-2469R
References: 38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
39968: Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530
39974: Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814
39975: Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775
運輸方式：凍存運輸
細胞類型：其他細胞類型
生長狀態(tài)：貼壁生長
是否是腫瘤細胞：0
物種來源：小鼠
ATCC Number：CRL-2469?
數量：大量
器官來源：大腦
品系：C3H/HeJ
細胞形態(tài)：單核細胞/巨噬細胞
規(guī)格：10mg Designations: EOC 20
Depositors: ?WS Walker
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus deposited as mouse
Morphology:macrophage
Source: Organ: brain
Strain: C3H/HeJ
Cell Type: microglia;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:colony stimulating factor 1 (CSF-1R, CD115)
Antigen Expression:CD11b/CD18 (Mac-1) +, Mac-2 +, Mac-3 +, CD80 (B7-1) +, CD45 +, Ly-6C +, MHC Class I +, MHC Class II +, CD115 (colony stimulating factor 1 receptor (CSF-1R)) +, FcR +, F4/80 +/-, CD86 (B7.2) - [39974 ]
Age: 10 days juvenile
Gender: female
Comments:This is an immortalized cell line derived from the brain of an apparently normal 10 day old mouse [PubMed: 8550814]. Cells were cloned in soft agar in the presence of CSF-1 and expanded on microcarrier beads. Beads were transferred to culture dishes and were subsequently passaged by scraping. The cell line is dependent on growth factor colony stimulating factor 1 (CSF-1). Conditioned medium is made from LADMAC cells (ATCC CRL-2420?) as a source of CSF-1.The cells exhibit phagocytic activity. These cells constitutively expressed high levels of major histocompatibility complex (MHC) class II antigens and expression was upregulated by recombinant murine interferon-gamma. The cells may be used to characterize the role of brain macrophages.C3H/HeJ strain is defective in TLR4 (toll-like receptor 4)
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove 75% of the media. Scrape off the attached cells and transfer into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
purified RNA:ATCC CRL-2469R
References: 38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247
39968: Walker WS. Establishment of mononuclear phagocyte cell lines. J. Immunol. Methods 174: 25-31, 1994. PubMed: 8083530
39974: Walker WS, et al. Mouse microglial cell lines differing in constitutive and interferon-gamma-inducible antigen-presenting activities for naive and memory CD4+ and CD8+ T cells. J. Neuroimmunol. 63: 163-174, 1995. PubMed: 8550814
39975: Askew D, Walker WS. Alloantigen presentation to naive CD8+ T cells by mouse microglia: evidence for a distinct phenotype based on expression of surface-associated and soluble costimulatory molecules. Glia 18: 118-128, 1996. PubMed: 8913775
