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細胞形態(tài)：淋巴樣
免疫類型：IgM
數(shù)量：大量
是否是腫瘤細胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
ATCC Number：HB-206?
運輸方式：凍存運輸
規(guī)格：0.5mg Designations: TE15
Depositors: ?BF Haynes
Isotype: IgM
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human thymic epithelium (Hassall's bodies)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with human thymic stroma.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with granular foci among the cellular swirls of Hassall's bodies.
It defines an antigen that is acquired between 14 and 16 weeks of fetal gestation, and marks the terminal stages of epidermal keratinocyte maturation.
The antibody binds specifically to granular foci among the cellular swirls of most Hassall's bodies and to stratum corneum of skin epidermis and foreskin.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1637: Lobach DF, et al. The human thymic microenvironment. Phenotypic characterization of Hassall's bodies with the use of monoclonal antibodies. J. Immunol. 134: 250-257, 1985. PubMed: 2578044