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平臺編號：bio-68480
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國際編號：CRL-1607?
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細胞信息： 6-23 (Clone 6)
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務費用：
加載中…… - 訂購
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注意事項：僅用于科學研究或者工業(yè)應用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產品信息以出庫為準）
6-23 (Clone 6)
ATCC Number：CRL-1607?
相關疾?。浩渌膊?br /> 運輸方式：凍存運輸
生長狀態(tài)：貼壁生長
細胞類型：其他細胞類型
器官來源：甲狀腺
是否是腫瘤細胞：0
物種來源：大鼠
數(shù)量：大量
規(guī)格：48T Designations: 6-23 (Clone 6)
Depositors: ?R Gagel, FN Zeytinoglu
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:
Source: Organ: thyroid
Disease: medullary thyroid carcinoma
Cell Type: C cell;
Cellular Products:neurotensin; calcitonin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Secretion of neurotensin and calcitonin is stimulated by calcium, KCl, glucagon and catecholamines.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; horse serum, 15%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 minute, remove trypsin EDTA, add 0.3 ml of 0.25% trypsin and let the culture sit at 37C for 6 minutes.
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 10 to 12 days.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 1085: Zeytinoglu FN, et al. Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. Morphological studies of the tumor and cells in culture. Endocrinology 107: 509-515, 1980. PubMed: 6248326
1088: Zeytinoglu FN, et al. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc. Natl. Acad. Sci. USA 77: 3741-3745, 1980. PubMed: 6932046
相關疾?。浩渌膊?br /> 運輸方式：凍存運輸
生長狀態(tài)：貼壁生長
細胞類型：其他細胞類型
器官來源：甲狀腺
是否是腫瘤細胞：0
物種來源：大鼠
數(shù)量：大量
規(guī)格：48T Designations: 6-23 (Clone 6)
Depositors: ?R Gagel, FN Zeytinoglu
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:
Source: Organ: thyroid
Disease: medullary thyroid carcinoma
Cell Type: C cell;
Cellular Products:neurotensin; calcitonin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Secretion of neurotensin and calcitonin is stimulated by calcium, KCl, glucagon and catecholamines.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 85%; horse serum, 15%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:10 to 1:20 is recommended
Medium Renewal: 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 minute, remove trypsin EDTA, add 0.3 ml of 0.25% trypsin and let the culture sit at 37C for 6 minutes.
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 10 to 12 days.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 1085: Zeytinoglu FN, et al. Establishment of a calcitonin-producing rat medullary thyroid carcinoma cell line. Morphological studies of the tumor and cells in culture. Endocrinology 107: 509-515, 1980. PubMed: 6248326
1088: Zeytinoglu FN, et al. Characterization of neurotensin production by a line of rat medullary thyroid carcinoma cells. Proc. Natl. Acad. Sci. USA 77: 3741-3745, 1980. PubMed: 6932046
