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平臺編號：bio-68481
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國際編號：CRL-2554?
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細(xì)胞信息： Panc 02.13
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Panc 02.13
年限：64 years
是否是腫瘤細(xì)胞：0
物種來源：人
數(shù)量：大量
ATCC Number：CRL-2554?
相關(guān)疾?。合侔?br /> 細(xì)胞形態(tài)：上皮樣
運(yùn)輸方式：凍存運(yùn)輸
器官來源：胰腺
生長狀態(tài)：貼壁生長
規(guī)格：0.2ml Designations: Panc 02.13
Depositors: ?EM Jaffee
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:epithelial
Source: Organ: pancreas
Disease: adenocarcinoma
Cellular Products:cytokeratins 7 and 18 [50655 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Oncogene:K-ras + (wild-type)
Antigen Expression:MHC class I +; MHC class II - [50655 ]
Blood type A; Rh+
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,12
D5S818: 12
D7S820: 7,11
THO1: 6,9.3
TPOX: 8
vWA: 16,18
Age: 64 years
Gender: female
Ethnicity: White
Comments:Panc 02.13 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.
The cells have a reported plating efficiency of 100%. [50655 ]
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 33.9 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602
是否是腫瘤細(xì)胞：0
物種來源：人
數(shù)量：大量
ATCC Number：CRL-2554?
相關(guān)疾?。合侔?br /> 細(xì)胞形態(tài)：上皮樣
運(yùn)輸方式：凍存運(yùn)輸
器官來源：胰腺
生長狀態(tài)：貼壁生長
規(guī)格：0.2ml Designations: Panc 02.13
Depositors: ?EM Jaffee
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens deposited as human
Morphology:epithelial
Source: Organ: pancreas
Disease: adenocarcinoma
Cellular Products:cytokeratins 7 and 18 [50655 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Oncogene:K-ras + (wild-type)
Antigen Expression:MHC class I +; MHC class II - [50655 ]
Blood type A; Rh+
DNA Profile (STR):Amelogenin: X
CSF1PO: 12
D13S317: 8,11
D16S539: 9,12
D5S818: 12
D7S820: 7,11
THO1: 6,9.3
TPOX: 8
vWA: 16,18
Age: 64 years
Gender: female
Ethnicity: White
Comments:Panc 02.13 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.
The cells have a reported plating efficiency of 100%. [50655 ]
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 33.9 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602
