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平臺編號：bio-68485
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國際編號：CRL-10762?
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細(xì)胞信息： CTLA4 Ig-24
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
CTLA4 Ig-24
是否是腫瘤細(xì)胞：0
物種來源：倉鼠
生長狀態(tài)：貼壁生長
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
ATCC Number：CRL-10762?
細(xì)胞形態(tài)：上皮樣
器官來源：卵巢
規(guī)格：0.1ml Designations: CTLA4 Ig-24
Depositors: ?Bristol-Myers Squibb Co.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Cricetulus griseus
Morphology:epithelial
Source: Organ: ovary
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: female
Comments:The line was constructed by transfecting CHO cells with a construct (CTLA4Ig) containing the sequence for the extracellular domain of the human CTLA-4 gene fused to the sequence for the hinge, CH2 and CH3 regions of human IgC.gamma.1 domain.The cells express the fusion protein (CTLA4Ig).
The cells express the fusion protein (CTLA4Ig).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 0.2 mM proline, 0.001 mM methotrexate, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
References: 4548: Linsley PS. Chimeric CTLA4 receptor and methods for its use. US Patent 5,434,131 dated Jul 18 1995
物種來源：倉鼠
生長狀態(tài)：貼壁生長
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
ATCC Number：CRL-10762?
細(xì)胞形態(tài)：上皮樣
器官來源：卵巢
規(guī)格：0.1ml Designations: CTLA4 Ig-24
Depositors: ?Bristol-Myers Squibb Co.
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Cricetulus griseus
Morphology:epithelial
Source: Organ: ovary
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: female
Comments:The line was constructed by transfecting CHO cells with a construct (CTLA4Ig) containing the sequence for the extracellular domain of the human CTLA-4 gene fused to the sequence for the hinge, CH2 and CH3 regions of human IgC.gamma.1 domain.The cells express the fusion protein (CTLA4Ig).
The cells express the fusion protein (CTLA4Ig).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 0.2 mM proline, 0.001 mM methotrexate, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin solution for 1 to 2 minutes, remove trypsin and let the culture sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
References: 4548: Linsley PS. Chimeric CTLA4 receptor and methods for its use. US Patent 5,434,131 dated Jul 18 1995
