-
平臺(tái)編號(hào)：bio-68492
-
國(guó)際編號(hào)：CRL-13011?
-
細(xì)胞信息： HeLa NR1
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
HeLa NR1
年限：31 years
ATCC Number：CRL-13011?
相關(guān)疾病：腺癌
器官來(lái)源：宮頸
數(shù)量：大量
細(xì)胞形態(tài)：上皮樣
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：48T Designations: HeLa NR1
Depositors: ?United States Government - DHHS
Biosafety Level:2 [cells contain papova and SV40 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: cervix
Disease: adenocarcinoma
Cell Type: HeLa cells transfected with plasmid pSV2neoNR101
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Reverse Transcript:Y
Age: 31 years
Gender: female
Ethnicity: Black
HeLa Markers: Y
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.5 mg/ml G418 and 10% fetal bovine serum
Temperature: 37.0°C
Subculturing: Protocol:
Subcultivation Ratio: 1:6 to 1:10
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-12510
References: 61432: Parker JE, Kiel JL. Nitrate reductase-transfected HeLa cells for cancer and microwave bioeffects research. US Patent 6,326,196 dated Dec 4 2001
ATCC Number：CRL-13011?
相關(guān)疾病：腺癌
器官來(lái)源：宮頸
數(shù)量：大量
細(xì)胞形態(tài)：上皮樣
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：48T Designations: HeLa NR1
Depositors: ?United States Government - DHHS
Biosafety Level:2 [cells contain papova and SV40 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: cervix
Disease: adenocarcinoma
Cell Type: HeLa cells transfected with plasmid pSV2neoNR101
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Reverse Transcript:Y
Age: 31 years
Gender: female
Ethnicity: Black
HeLa Markers: Y
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.5 mg/ml G418 and 10% fetal bovine serum
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: 1:6 to 1:10
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CRL-12510
References: 61432: Parker JE, Kiel JL. Nitrate reductase-transfected HeLa cells for cancer and microwave bioeffects research. US Patent 6,326,196 dated Dec 4 2001
