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平臺編號：bio-68497
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國際編號：CRL-12135?
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細胞信息： Con A - C1 - VICK
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
Con A - C1 - VICK
運輸方式：凍存運輸
數(shù)量：大量
器官來源：脾
生長狀態(tài)：懸浮生長
年限：42 week old
ATCC Number：CRL-12135?
細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：其他
細胞形態(tài)：淋巴樣
規(guī)格：0.1ml Designations: Con A - C1 - VICK
Depositors: ?USDA/ARS
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, multicell aggregates, clusters in suspension
Organism: Gallus gallus deposited as chicken
Morphology:lymphoblast
Source: Organ: spleen
Cell Type: T lymphocyte; transformed with reticuloendotheliosis virus (ATCC VR-770 )
Cellular Products:granulocyte colony stimulating factor (G-CSF)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 42 week old
Gender: female
Comments:Animals were immunized with Salmonella enteritidis.
Spleens were harvested and T cells were isolated.
T cells were incubated with Concanavalin A and subsequently transformed with Avian reticuloendotheliosis virus (REV-T with CSV)(ATCC VR-770 ).
Two immortal cell lines were established, CON A-C1-VICK (ATCC CRL-12135 ) and ConA-B1-VICK (ATCC CRL-12357 ).
Both cell lines produce granulocyte colony stimulating factor (G-CSF).
When cultured in vitro, the cell lines produce and secrete immune lymphokines that may be administered to fowl to increase their resistance to infections.
The cell lines produce lymphokines which, following administration into either 18 day old chick embryos or day of hatch chicks, prevents extraintestinal Salmonella infection.
Neither the lymphokine or cell line induces viral pathogenesis in chickens.
For lymphokine production, remove cells from maintenance medium, wash in serum-free RPMI, culture at 5 x 10 exp6 cells/ml in serum-free RPMI containing 0.0075 mg/ml Concanavalin A (ConA) for 72 hours.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Temperature: 37.0°C
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: Complete culture medium, 90%; additional fetal bovine serum, 5%; DMSO, 5%.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 43306: Kogut MH, et al. Method to produce granulocyte colony stimulating factor from immortalized avian T lymphocytes and method to produce immortalized cells. US Patent 5,691,200 dated Nov 25 1997
數(shù)量：大量
器官來源：脾
生長狀態(tài)：懸浮生長
年限：42 week old
ATCC Number：CRL-12135?
細胞類型：其他細胞類型
是否是腫瘤細胞：0
物種來源：其他
細胞形態(tài)：淋巴樣
規(guī)格：0.1ml Designations: Con A - C1 - VICK
Depositors: ?USDA/ARS
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension, multicell aggregates, clusters in suspension
Organism: Gallus gallus deposited as chicken
Morphology:lymphoblast
Source: Organ: spleen
Cell Type: T lymphocyte; transformed with reticuloendotheliosis virus (ATCC VR-770 )
Cellular Products:granulocyte colony stimulating factor (G-CSF)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 42 week old
Gender: female
Comments:Animals were immunized with Salmonella enteritidis.
Spleens were harvested and T cells were isolated.
T cells were incubated with Concanavalin A and subsequently transformed with Avian reticuloendotheliosis virus (REV-T with CSV)(ATCC VR-770 ).
Two immortal cell lines were established, CON A-C1-VICK (ATCC CRL-12135 ) and ConA-B1-VICK (ATCC CRL-12357 ).
Both cell lines produce granulocyte colony stimulating factor (G-CSF).
When cultured in vitro, the cell lines produce and secrete immune lymphokines that may be administered to fowl to increase their resistance to infections.
The cell lines produce lymphokines which, following administration into either 18 day old chick embryos or day of hatch chicks, prevents extraintestinal Salmonella infection.
Neither the lymphokine or cell line induces viral pathogenesis in chickens.
For lymphokine production, remove cells from maintenance medium, wash in serum-free RPMI, culture at 5 x 10 exp6 cells/ml in serum-free RPMI containing 0.0075 mg/ml Concanavalin A (ConA) for 72 hours.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Temperature: 37.0°C
Subculturing: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: Complete culture medium, 90%; additional fetal bovine serum, 5%; DMSO, 5%.
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 43306: Kogut MH, et al. Method to produce granulocyte colony stimulating factor from immortalized avian T lymphocytes and method to produce immortalized cells. US Patent 5,691,200 dated Nov 25 1997
