運輸方式：凍存運輸
數(shù)量：大量
ATCC Number：CRL-2631?
相關疾病：其他疾病
生長狀態(tài)：懸浮生長
年限：adult
器官來源：外周血
細胞類型：B淋巴細胞
是否是腫瘤細胞：0
物種來源：人
細胞形態(tài)：淋巴樣
規(guī)格：0.5mg Designations: Toledo
Depositors: ?H Ben-Bassat
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens deposited as human
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1990
Antigen Expression:CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289 ]
DNA Profile (STR):D5S818:10,12
D7S820: 9,10
THO1: 8,9.3
TPOX: 8,11
vWA: 16,17
Amelogenin: X
CSF1PO: 12
D13S317:11
D16S539:9
Age: adult
Gender: female
Ethnicity: White
Comments:Toledo was established in 1990 from peripheral blood leukocytes (PBL) of a patient that originally had a diffuse large cell lymphoma (DLCL). [53289 ]
Following high-dose chemotherapy and bone marrow transplantation, the patient subsequently developed a lymphoma in the brain. [53289 ]
Though the morphology of the cells resembles Burkitt's lymphoma, the cells lack the typical chromosomal translocations of Burkitt's lymphoma. The karyotype does exhibit multiple chromosomal aberrations. [53289 ]
The cells do not express surface or cytoplasmic immunoglobulin. [53289 ]
The cells are negative for Epstein-Barr virus. [53289 ]
This cell line is a model system for studying non-Hodgkin lymphomas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 3 X 10(5) and 3 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 24 to 30 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
