器官來源：外周血
生長狀態(tài)：懸浮生長
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
年限：19 years
細(xì)胞類型：T淋巴細(xì)胞
細(xì)胞形態(tài)：淋巴樣
是否是腫瘤細(xì)胞：0
物種來源：人
ATCC Number：CRL-1552?
相關(guān)疾?。喊籽?br />
規(guī)格：0.2ml Designations: MOLT-3
Depositors: ?J Minowada
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: T lymphoblast;
Cellular Products:terminal deoxynucleotidyl transferase (TdT) activity is high [22735 ]
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Antigen Expression:CD1 (65%), CD4 (66%), CD5 (97%), CD7 (97%)
Cytogenetic Analysis:This is a hypertetraploid human cell line. The modal chromosome number was 97, occurring in 48% of cells. The rate of cells with higher ploidies was 2%. Three markers were common to all cells. They are: ?del(6) (q21), t(7;7), and an unidentifiable M5. The der(6) generally had three copies per cell, and the i(7q), two copies. The majority of normal chromosomes had 4 copies per cell. N8, N17, N19 and N20 had more than 4 copies in many cells. The X and Y chromosomes were two copies each per cell. Neither HSR chromosomes nor DM's were detected.
Isoenzymes: AK-1, 0
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 0
Age: 19 years
Gender: male
Comments:No immunoglobulin or Epstein-Barr virus is detectable. [22524 ]
This line was established from cells taken from a patient in relapse. [22524 ]
The patient had received prior multidrug chemotherapy. [22524 ]
MOLT-3 was derived from the same patient as the MOLT-4 cell line (ATCC CRL-1582 ). [22524 ]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures are maintained by addition or replacement of fresh medium. Establish new cultures at 5 X 10(5) viable cells/ml and subculture at between 1 and 2 X 10(6) cells/ml.
Medium Renewal: Every 3 to 4 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derived from same individual:ATCC CRL-1582
References: 22524: Minowada J, et al. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891-895, 1972. PubMed: 4567231
22735: Mertelsmann R, et al. T-cell growth factor (interleukin 2) and terminal transferase activity in human leukemias and lymphoblastic cell lines. Blut 43: 99-103, 1981. PubMed: 6942897
