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平臺編號：bio-68503
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國際編號：ATCC CRL-2038
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細胞信息： M-1
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規(guī)格：frozen
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用途：ATCC原裝細胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
M-1
運輸方式：凍存運輸
器官來源：腎皮質(zhì)
細胞形態(tài)：上皮樣
數(shù)量：大量
生長狀態(tài)：貼壁生長
組織來源：collecting duct
ATCC Number：CRL-2038?
是否是腫瘤細胞：0
物種來源：轉(zhuǎn)基因小鼠
規(guī)格：0.1ml Designations: M-1
Depositors: ?G Fejes-Toth
Biosafety Level:2 [Cells contain SV-40 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus , transgenic for SV40 early region deposited as mouse, transgenic for SV40 early region
Morphology:epithelial
Source: Organ: kidney, cortex
Tissue: collecting duct
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host (Roche Transfection Reagents )
Comments:The M-1 cell line was established from normal renal tissue taken from a mouse transgenic for the SV40 early region (tg(SV40E)Bri7).
The cells retain many characteristics of cortical collecting duct (CCD) cells including morphology and CCD antigens.
Most cell lines cloned from M-1 exhibit characteristics of either intercalated cells (ICC) or principle cells (PC) of the CCD.
5 to 10% of the cells exhibit a dual PC - ICC phenotype.
When grown on permeable supports, the cells develop a lumen negative transepithelial potential difference.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES , 0.5 mM sodium pyruvate and 1.2 g/L sodium bicarbonate supplemented with 0.005 mM dexamethasone and 5% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 23331: Fejes-Toth G, Naray-Fejes-Toth A. Differentiation of renal beta-intercalated cells to alpha-intercalated and principal cells in culture. Proc. Natl. Acad. Sci. USA 89: 5487-5491, 1992. PubMed: 1608958
23523: Stoos BA, et al. Characterization of a mouse cortical collecting duct cell line. Kidney Int. 39: 1168-1175, 1991. PubMed: 1654478
器官來源：腎皮質(zhì)
細胞形態(tài)：上皮樣
數(shù)量：大量
生長狀態(tài)：貼壁生長
組織來源：collecting duct
ATCC Number：CRL-2038?
是否是腫瘤細胞：0
物種來源：轉(zhuǎn)基因小鼠
規(guī)格：0.1ml Designations: M-1
Depositors: ?G Fejes-Toth
Biosafety Level:2 [Cells contain SV-40 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Mus musculus , transgenic for SV40 early region deposited as mouse, transgenic for SV40 early region
Morphology:epithelial
Source: Organ: kidney, cortex
Tissue: collecting duct
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:transfection host (Roche Transfection Reagents )
Comments:The M-1 cell line was established from normal renal tissue taken from a mouse transgenic for the SV40 early region (tg(SV40E)Bri7).
The cells retain many characteristics of cortical collecting duct (CCD) cells including morphology and CCD antigens.
Most cell lines cloned from M-1 exhibit characteristics of either intercalated cells (ICC) or principle cells (PC) of the CCD.
5 to 10% of the cells exhibit a dual PC - ICC phenotype.
When grown on permeable supports, the cells develop a lumen negative transepithelial potential difference.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES , 0.5 mM sodium pyruvate and 1.2 g/L sodium bicarbonate supplemented with 0.005 mM dexamethasone and 5% fetal bovine serum
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 23331: Fejes-Toth G, Naray-Fejes-Toth A. Differentiation of renal beta-intercalated cells to alpha-intercalated and principal cells in culture. Proc. Natl. Acad. Sci. USA 89: 5487-5491, 1992. PubMed: 1608958
23523: Stoos BA, et al. Characterization of a mouse cortical collecting duct cell line. Kidney Int. 39: 1168-1175, 1991. PubMed: 1654478
