-
平臺編號：bio-68510
-
國際編號：CRL-2773?
-
細胞信息： WBE
-
規(guī)格：frozen
-
用途：ATCC原裝細胞
-
服務(wù)費用：
加載中…… - 訂購
-
注意事項：僅用于科學研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準）
WBE
年限：24 hours post fertilization embryo
運輸方式：凍存運輸
器官來源：胚胎
細胞類型：上皮細胞
細胞形態(tài)：上皮樣
是否是腫瘤細胞：0
物種來源：其他
數(shù)量：大量
ATCC Number：CRL-2773?
生長狀態(tài)：貼壁生長
規(guī)格：0.2ml Designations: WBE
Depositors: ?M Westerman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Morone chrysops
Morphology:epithelial
Source: Organ: embryo
Cell Type: epithelial
Cellular Products:keratin [90251 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: San Diego California,
Isolation date: February 22, 2001
Cytogenetic Analysis:Aneuploid. Range = 44 to 49. The chromosme morphology was normal for bass with all telocentric and acrocentric chromosomes. No dicentric or ring chromosomes were observed. [90251 ]
Age: 24 hours post fertilization embryo
Gender: unknown
Comments:These cells are useful for the study of host-pathogen interactions.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 31.0°C
Subculturing: Protocol:
Subcultivation Ratio: 1:4 to 1:6 weekly
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 90251: Shimizu C, et al. Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cell. Dev. Biol. Anim. 39: 29-35, 2003. PubMed: 12892524
運輸方式：凍存運輸
器官來源：胚胎
細胞類型：上皮細胞
細胞形態(tài)：上皮樣
是否是腫瘤細胞：0
物種來源：其他
數(shù)量：大量
ATCC Number：CRL-2773?
生長狀態(tài)：貼壁生長
規(guī)格：0.2ml Designations: WBE
Depositors: ?M Westerman
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Morone chrysops
Morphology:epithelial
Source: Organ: embryo
Cell Type: epithelial
Cellular Products:keratin [90251 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: San Diego California,
Isolation date: February 22, 2001
Cytogenetic Analysis:Aneuploid. Range = 44 to 49. The chromosme morphology was normal for bass with all telocentric and acrocentric chromosomes. No dicentric or ring chromosomes were observed. [90251 ]
Age: 24 hours post fertilization embryo
Gender: unknown
Comments:These cells are useful for the study of host-pathogen interactions.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 31.0°C
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 31?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 31?C.
Subcultivation Ratio: 1:4 to 1:6 weekly
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 90251: Shimizu C, et al. Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cell. Dev. Biol. Anim. 39: 29-35, 2003. PubMed: 12892524
