-
平臺(tái)編號(hào)：bio-68512
-
國際編號(hào)：CRL-2750?
-
細(xì)胞信息： PHL
-
規(guī)格：frozen
-
用途：ATCC原裝細(xì)胞
-
服務(wù)費(fèi)用：
加載中…… - 訂購
-
注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
PHL
年限：newly hatched larvae larva
數(shù)量：大量
器官來源：其他
細(xì)胞類型：上皮細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：其他
ATCC Number：CRL-2750?
細(xì)胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.2ml Designations: PHL
Depositors: ?NC Bols
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Clupea pallasi
Morphology:epithelial
Source: Organ: larvae
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Waterloo Ontario, Canada
Virus Susceptibility:Viral hemorrhagic septicemia virus
Cytogenetic Analysis:The cells retain a diploid karyotype beyond 65 population doublings.
Age: newly hatched larvae larva
Comments:The PHL (Pacific Herring Larva) cell line was established from newly hatched larvae by explant culture. These cells retain a diploid karotype after 65 population doublings. PHL cells are susceptible to infection by the North American strain of viral hemorrhagic septicemia (VHS) virus, and are sensitive to the cytotoxic effects of naphthalene, a common environmental contaminant. This cell line is useful for investigation of the toxicity of naphthalene and other petroleum components to juvenile herring. In addition, it can be used to study the VHS virus.
Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Atmosphere: air, 100%
Temperature: 21.0°C; (Max. 21.0 °C, Min. 18.0°C)
Subculturing: Protocol:
Interval: Maintain cultures at a cell concentration between 4 X 10(3) and 5 X 10(4) cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: about 34 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 70352: Ganassin RC, et al. Development and characterization of a cell line from Pacific herring, Clupea harengus pallasi, sensitive to both naphthalene cytotoxicity and infection by viral hemorrhagic septicemia virus. Cell Biol. Toxicol. 15: 299-309, 1999. PubMed: 10813363
數(shù)量：大量
器官來源：其他
細(xì)胞類型：上皮細(xì)胞
是否是腫瘤細(xì)胞：0
物種來源：其他
ATCC Number：CRL-2750?
細(xì)胞形態(tài)：上皮樣
生長狀態(tài)：貼壁生長
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.2ml Designations: PHL
Depositors: ?NC Bols
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Clupea pallasi
Morphology:epithelial
Source: Organ: larvae
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Waterloo Ontario, Canada
Virus Susceptibility:Viral hemorrhagic septicemia virus
Cytogenetic Analysis:The cells retain a diploid karyotype beyond 65 population doublings.
Age: newly hatched larvae larva
Comments:The PHL (Pacific Herring Larva) cell line was established from newly hatched larvae by explant culture. These cells retain a diploid karotype after 65 population doublings. PHL cells are susceptible to infection by the North American strain of viral hemorrhagic septicemia (VHS) virus, and are sensitive to the cytotoxic effects of naphthalene, a common environmental contaminant. This cell line is useful for investigation of the toxicity of naphthalene and other petroleum components to juvenile herring. In addition, it can be used to study the VHS virus.
Propagation: ATCC complete growth medium: Leibovitz's L-15 medium with 2 mM L-glutamine, 85%; fetal bovine serum, 15%
Atmosphere: air, 100%
Temperature: 21.0°C; (Max. 21.0 °C, Min. 18.0°C)
Subculturing: Protocol:
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 21?C.
Interval: Maintain cultures at a cell concentration between 4 X 10(3) and 5 X 10(4) cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: about 34 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 70352: Ganassin RC, et al. Development and characterization of a cell line from Pacific herring, Clupea harengus pallasi, sensitive to both naphthalene cytotoxicity and infection by viral hemorrhagic septicemia virus. Cell Biol. Toxicol. 15: 299-309, 1999. PubMed: 10813363
