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平臺編號：bio-68513
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國際編號：CRL-2173?
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細(xì)胞信息： FIGR
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
FIGR
ATCC Number：CRL-2173?
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b
是否是腫瘤細(xì)胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：48T Designations: FIGR
Depositors: ?JE Bodwell
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against glucocorticoid receptor of mouse and rat
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with the glucorticoid receptor (GR) of mouse and rat, but does not react with human GR.
The epitope bound on the mouse receptor is at residues 395 - 411.
The antibody produced by FIGR appears to be equivalent to that produced by the BUGR2 hybridoma in that the two antibodies will cross inhibit, and both bing to the same 16000 dalton tryptic fragment of GR.
The antibody is useful for immunopurification and Western blotting applications.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; iron supplemented bovine calf serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23409: Bodwell JE, et al. Identification of phosphorylated sites in the mouse glucocorticoid receptor. J. Biol. Chem. 266: 7549-7555, 1991. PubMed: 2019585
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG2b
是否是腫瘤細(xì)胞：0
物種來源：小鼠
生長狀態(tài)：懸浮生長
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：48T Designations: FIGR
Depositors: ?JE Bodwell
Isotype: IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against glucocorticoid receptor of mouse and rat
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with the glucorticoid receptor (GR) of mouse and rat, but does not react with human GR.
The epitope bound on the mouse receptor is at residues 395 - 411.
The antibody produced by FIGR appears to be equivalent to that produced by the BUGR2 hybridoma in that the two antibodies will cross inhibit, and both bing to the same 16000 dalton tryptic fragment of GR.
The antibody is useful for immunopurification and Western blotting applications.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; iron supplemented bovine calf serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 23409: Bodwell JE, et al. Identification of phosphorylated sites in the mouse glucocorticoid receptor. J. Biol. Chem. 266: 7549-7555, 1991. PubMed: 2019585
