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平臺(tái)編號(hào)：bio-68516
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國(guó)際編號(hào)：CRL-1955?
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細(xì)胞信息： P-tyr-1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
P-tyr-1
數(shù)量：大量
ATCC Number：CRL-1955?
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
規(guī)格：0.1ml Designations: P-tyr-1
Depositors: ?JK Klarlund
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against phosphotyrosine
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with aminobenzylphosphonate conjugated to hemocyanin.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with phosphotyrosine and proteins containing phosphotyrosine.
The epitope recognized by P-tyr-1 appears to be on the phenyl phosphate group of phosphotyrosine.
The antibody does not react with phosphothreonine or phosphoserine, and is useful in immunoprecipitation and western blot assays.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22627: Klarlund JK. Transformation of cells by an inhibitor of phosphatases acting on phosphotyrosine in proteins. Cell 41: 707-717, 1985. PubMed: 2408760
22706: Klarlund JK, et al. Numerous proteins phosphorylated on tyrosine and enhanced tyrosine kinase activities in vanadate-treated NIH 3T3 fibroblasts. Biochim. Biophys. Acta 971: 112-120, 1988. PubMed: 3408742
ATCC Number：CRL-1955?
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
是否是腫瘤細(xì)胞：0
物種來(lái)源：小鼠
規(guī)格：0.1ml Designations: P-tyr-1
Depositors: ?JK Klarlund
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against phosphotyrosine
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with aminobenzylphosphonate conjugated to hemocyanin.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with phosphotyrosine and proteins containing phosphotyrosine.
The epitope recognized by P-tyr-1 appears to be on the phenyl phosphate group of phosphotyrosine.
The antibody does not react with phosphothreonine or phosphoserine, and is useful in immunoprecipitation and western blot assays.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22627: Klarlund JK. Transformation of cells by an inhibitor of phosphatases acting on phosphotyrosine in proteins. Cell 41: 707-717, 1985. PubMed: 2408760
22706: Klarlund JK, et al. Numerous proteins phosphorylated on tyrosine and enhanced tyrosine kinase activities in vanadate-treated NIH 3T3 fibroblasts. Biochim. Biophys. Acta 971: 112-120, 1988. PubMed: 3408742
