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細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：其他
生長狀態(tài)：懸浮生長
ATCC Number：CRL-2758?
細(xì)胞形態(tài)：淋巴樣
運(yùn)輸方式：凍存運(yùn)輸
數(shù)量：大量
器官來源：外周血
規(guī)格：0.5mg Designations: 28S.3
Depositors: ?NW Miller
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Ictalurus punctatus
Morphology:lymphoblast


Source: Organ: peripheral blood
Cell Type: lymphocyte T lymphoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1996
Cytogenetic Analysis:diploid
Comments:The 28S.3 cell line, developed in 1996, is an immortal T cell line spontaneously derived from a long term culture of peripheral blood from a normal channel catfish. The cells were subsequently cloned by limiting dilution. The cells are maintained in vitro without restimulation, feeder cells, or exogenous factors. The cells are immunoglobulin negative [PubMed: 9809582].The cells constitutively produce a supernatant factor(s) with growth promoting activity [PubMed: 9809582].
Propagation: ATCC complete growth medium: A 1:1 mixture of Leibovitz's L-15 medium with 2 mM L-glutamine, and AIM-V Medium adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 87.5%; double distilled water, 10%; heat-inactivated Catfish serum, 2.5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 27.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.
Interval: Maintain cell density between 5 X 10(5) and 5 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 61272: Wilson MR, et al. T-cell receptors in channel catfish: structure and expression of TCR alpha and beta genes. Mol. Immunol. 35: 545-557, 1998. PubMed: 9809582