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F18 AF1
    F18 AF1
  • 平臺編號:bio-68523
  • 國際編號:HB-8208?
  • 細(xì)胞信息: F18 AF1
  • 規(guī)格:frozen
  • 用途:ATCC原裝細(xì)胞
  • 服務(wù)費(fèi)用:
    加載中……
  • 訂購
  • 注意事項(xiàng):僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療,非藥用,非食用(產(chǎn)品信息以出庫為準(zhǔn))

數(shù)量:大量
是否是腫瘤細(xì)胞:0
物種來源:小鼠
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG1
生長狀態(tài):懸浮生長
ATCC Number:HB-8208?
運(yùn)輸方式:凍存運(yùn)輸
規(guī)格:48T Designations: F18 AF1
Depositors: ?Amgen, Inc.
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human colony stimulating factor subclass I (CSF-I)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with purified CSF-I.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
CSF-I was purified from medium conditioned by MIA PaCa-2 (ATCC CRL-1420 ).
Propagation: ATCC complete growth medium: HB101 medium (Hana Biologics), 99%; heat-inactivated fetal bovine serum, 1%

Subculturing: Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3514: Nicolson M. Hybridoma tumor cell lines and their monoclonal antibodies to human colony stimulating factor subclass number 1. US Patent 4,504,586 dated Mar 12 1985
22162: . . Hybridoma 2: 137, 1983.

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