數(shù)量:大量
是否是腫瘤細(xì)胞:0
物種來源:小鼠
細(xì)胞形態(tài):淋巴樣
免疫類型:IgG1
生長狀態(tài):懸浮生長
ATCC Number:HB-8208?
運(yùn)輸方式:凍存運(yùn)輸
規(guī)格:48T Designations: F18 AF1
Depositors: ?Amgen, Inc.
Isotype: IgG1
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma)
Morphology:lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against human colony stimulating factor subclass I (CSF-I)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Comments:Animals were immunized with purified CSF-I.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
CSF-I was purified from medium conditioned by MIA PaCa-2 (ATCC CRL-1420 ).
Propagation: ATCC complete growth medium: HB101 medium (Hana Biologics), 99%; heat-inactivated fetal bovine serum, 1%
Subculturing: Medium Renewal: Twice per week
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 3514: Nicolson M. Hybridoma tumor cell lines and their monoclonal antibodies to human colony stimulating factor subclass number 1. US Patent 4,504,586 dated Mar 12 1985
22162: . . Hybridoma 2: 137, 1983.