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生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
免疫類型：IgG1 and IgG2b
ATCC Number：CRL-2654?
是否是腫瘤細(xì)胞：0
物種來源：小鼠
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.5mg Designations: 147-67C6
Depositors: ?National Cancer Institute
Isotype: IgG1 and IgG2b
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology:lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against a synthetic v-ras K oncogene peptide
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments:Animals were immunized with a synthetic peptide with the sequence YTLVREIRQYRLKKESKEEKTPGC corresponding to peptides 157-180 of the v-ras K oncogene.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts with the synthetic v-ras K oncogene peptide. In ELISA testing, the antibody weakly detects the homologous region in the ras H sequence. In immunoblot testing a serum protein of approximately 45 kd is detected.
The IgG1 and IgG2b reactivities of the antibody with the v-ras K peptide (157-180) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.
Maintain cell density between 2 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020