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平臺編號：bio-68539
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國際編號：HTB-24?
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細(xì)胞信息： MDA-MB-157
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
MDA-MB-157
數(shù)量：大量
細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：0
物種來源：人
運輸方式：凍存運輸
年限：44 years adult
ATCC Number：HTB-24?
相關(guān)疾?。浩渌膊?br /> 器官來源：乳房
生長狀態(tài)：貼壁生長
組織來源：medulla
規(guī)格：0.1ml Designations: MDA-MB-157
Depositors: ?R Cailleau
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: mammary gland; breast
Tissue: medulla
Disease: medulallary carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:Blood Type B; Rh-
DNA Profile (STR):Amelogenin: X
D13S317: 11
CSF1PO: 10
D16S539: 11
D5S818: 12
D7S820: 10, 11
THO1: 7 ,8
TPOX: 11, 9
vWA : 15
Cytogenetic Analysis:modal number =52 to 54; range = 52 to 69.
Most of the karyotypes had three normal X chromosomes. Normal chromosomes N1, N5, N13, N14, N15, N16, N19, and N22 were not found. Seventeen marker chromosomes were identified: t(10q14q) der(12)t(1;12)(q21;p13), der(7)t(7;?)(q22;?), del(1)(q11), t(3qter>3q21::10p15>10qter), 7q+, del(5)(q13), 19q+, t(10q;14q), der(4)t(1;4)(q21;q25), 9q+, 13p+, 15p+ unknown, unknown, unknown, del(6)(q21). Marker chromosome M1 is similar to that described by Q.V. Cruciger, et al., Cytogenet. Cell Genet. 17: 231, 1976.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 44 years adult
Gender: female
Ethnicity: Black
Comments:The cells express the WNT7B oncogene [PubMed: 8168088].
The method for derivation of this line was similar in detail to that for ATCC HTB-23.
Desmosomes, microvilli and tonofilaments were observed at boundaries between cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337
22182: Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.
22398: Young RK, et al. Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma. In Vitro 9: 239-245, 1974. PubMed: 4471183
22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247
22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088
26319: Cruciger QV, et al. Human breast carcinomas: marker chromosomes involving 1q in seven cases. Cytogenet. Cell Genet. 17: 231-235, 1976. PubMed: 1001030
細(xì)胞形態(tài)：上皮樣
是否是腫瘤細(xì)胞：0
物種來源：人
運輸方式：凍存運輸
年限：44 years adult
ATCC Number：HTB-24?
相關(guān)疾?。浩渌膊?br /> 器官來源：乳房
生長狀態(tài)：貼壁生長
組織來源：medulla
規(guī)格：0.1ml Designations: MDA-MB-157
Depositors: ?R Cailleau
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Homo sapiens
Morphology:epithelial
Source: Organ: mammary gland; breast
Tissue: medulla
Disease: medulallary carcinoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic:Yes
Antigen Expression:Blood Type B; Rh-
DNA Profile (STR):Amelogenin: X
D13S317: 11
CSF1PO: 10
D16S539: 11
D5S818: 12
D7S820: 10, 11
THO1: 7 ,8
TPOX: 11, 9
vWA : 15
Cytogenetic Analysis:modal number =52 to 54; range = 52 to 69.
Most of the karyotypes had three normal X chromosomes. Normal chromosomes N1, N5, N13, N14, N15, N16, N19, and N22 were not found. Seventeen marker chromosomes were identified: t(10q14q) der(12)t(1;12)(q21;p13), der(7)t(7;?)(q22;?), del(1)(q11), t(3qter>3q21::10p15>10qter), 7q+, del(5)(q13), 19q+, t(10q;14q), der(4)t(1;4)(q21;q25), 9q+, 13p+, 15p+ unknown, unknown, unknown, del(6)(q21). Marker chromosome M1 is similar to that described by Q.V. Cruciger, et al., Cytogenet. Cell Genet. 17: 231, 1976.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 44 years adult
Gender: female
Ethnicity: Black
Comments:The cells express the WNT7B oncogene [PubMed: 8168088].
The method for derivation of this line was similar in detail to that for ATCC HTB-23.
Desmosomes, microvilli and tonofilaments were observed at boundaries between cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
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Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal. - Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
- Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37C in air atmosphere.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337
22182: Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.
22398: Young RK, et al. Establishment of epithelial cell line MDA-MB-157 from metastatic pleural effusion of human breast carcinoma. In Vitro 9: 239-245, 1974. PubMed: 4471183
22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247
22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
23113: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088
26319: Cruciger QV, et al. Human breast carcinomas: marker chromosomes involving 1q in seven cases. Cytogenet. Cell Genet. 17: 231-235, 1976. PubMed: 1001030
