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平臺編號：bio-68543
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國際編號：CRL-2309?
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細(xì)胞信息： CCD 1106 KERTr
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
CCD 1106 KERTr
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：人
細(xì)胞形態(tài)：上皮樣
年限：133 days gestation
ATCC Number：CRL-2309?
器官來源：皮膚
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.1ml Designations: CCD 1106 KERTr
Depositors: ?L Vilner
Biosafety Level:2 [Cells may contain the Human Papilloma viral (HPV) sequences ]
Shipped: frozen
Medium & Serum: See Propagation Organism: Homo sapiens
Morphology:epithelial
Source: Organ: skin
Cell Type: keratinocyte; human papillomavirus 16 (HPV-16) E6/E7 transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Note: These cells are distributed for research purposes only. The American Type Culture Collection ( ATCC ) releases the line subject to the following: 1. CCD 1106 KERTr cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of the ATCC ; 2. Any proposed commercial use of this cell line must first be negotiated with the ATCC , P.O. Box 1549, Manassas VA 20108, telephone 703-365-2704, FAX 703-365-2730.
Oncogene:E6/E7 +
Antigen Expression:epithelial specific antigen
Age: 133 days gestation
Comments:Passage 2 cells were transformed with a retrovirus vector ( LXSN16E6E7 produced by ATCC CRL-2203 ) in the presence of polybrene.
This line stains positively with antibody for cytokeratin.
After 50 population doublings, the cells continue dividing and retain cuboidal morphology.
E6E7 sequences were detected by PCR in cells at passage 18.
Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.
Epithelial specific antigen was detected using antibody produced by the Ep16 hybridoma (ATCC HB-155 ).
Propagation: ATCC complete growth medium: These cells are grown in Keratinocyte-Serum Free Medium (Gibco 17005-042)supplemented with
Temperature: 37.0°C
Add fresh medium twice per week
Subculturing: Protocol: Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution. Allow the flasks to sit at room temperature (or incubate at 37C) until the cells detach. Neutralize the trypsin with fresh culture medium, centrifuge, resuspend cells in fresh culture medium and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Add fresh medium twice per week
Preservation: Freeze medium: Ham's F12 medium 85%; DMSO, 5%; FBS 10%
Storage temperature: liquid nitrogen vapor phase
References: 38275: Zabrenetzky V, et al. The isolation, immortalization and characterization of human fetal keratinocytes. In Vitro Cell. Dev. Biol. 33: Part II, p. 34A, 1997.
是否是腫瘤細(xì)胞：0
物種來源：人
細(xì)胞形態(tài)：上皮樣
年限：133 days gestation
ATCC Number：CRL-2309?
器官來源：皮膚
數(shù)量：大量
運(yùn)輸方式：凍存運(yùn)輸
規(guī)格：0.1ml Designations: CCD 1106 KERTr
Depositors: ?L Vilner
Biosafety Level:2 [Cells may contain the Human Papilloma viral (HPV) sequences ]
Shipped: frozen
Medium & Serum: See Propagation Organism: Homo sapiens
Morphology:epithelial
Source: Organ: skin
Cell Type: keratinocyte; human papillomavirus 16 (HPV-16) E6/E7 transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:Note: These cells are distributed for research purposes only. The American Type Culture Collection ( ATCC ) releases the line subject to the following: 1. CCD 1106 KERTr cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of the ATCC ; 2. Any proposed commercial use of this cell line must first be negotiated with the ATCC , P.O. Box 1549, Manassas VA 20108, telephone 703-365-2704, FAX 703-365-2730.
Oncogene:E6/E7 +
Antigen Expression:epithelial specific antigen
Age: 133 days gestation
Comments:Passage 2 cells were transformed with a retrovirus vector ( LXSN16E6E7 produced by ATCC CRL-2203 ) in the presence of polybrene.
This line stains positively with antibody for cytokeratin.
After 50 population doublings, the cells continue dividing and retain cuboidal morphology.
E6E7 sequences were detected by PCR in cells at passage 18.
Major Histocompatibility Complex class I or II molecules were not expressed on these cells, but PCR analyses revealed presence of the genes for directing synthesis of HLA antigens.
Epithelial specific antigen was detected using antibody produced by the Ep16 hybridoma (ATCC HB-155 ).
Propagation: ATCC complete growth medium: These cells are grown in Keratinocyte-Serum Free Medium (Gibco 17005-042)supplemented with
- 0.05 mg/ml bovine pituitary extract (BPE)
- 35 ng/ml human recombinant epidermal growth factor (EGF)
Temperature: 37.0°C
Add fresh medium twice per week
Subculturing: Protocol: Remove medium, rinse two times with cold 0.25% trypsin, 0.03% EDTA solution. Allow the flasks to sit at room temperature (or incubate at 37C) until the cells detach. Neutralize the trypsin with fresh culture medium, centrifuge, resuspend cells in fresh culture medium and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Add fresh medium twice per week
Preservation: Freeze medium: Ham's F12 medium 85%; DMSO, 5%; FBS 10%
Storage temperature: liquid nitrogen vapor phase
References: 38275: Zabrenetzky V, et al. The isolation, immortalization and characterization of human fetal keratinocytes. In Vitro Cell. Dev. Biol. 33: Part II, p. 34A, 1997.
