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平臺(tái)編號(hào)：bio-68546
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國(guó)際編號(hào)：CRL-8756?
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細(xì)胞信息： SC-1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
加載中…… - 訂購(gòu)
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類(lèi)或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫(kù)為準(zhǔn)）
SC-1
生長(zhǎng)狀態(tài)：懸浮生長(zhǎng)
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
ATCC Number：CRL-8756?
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類(lèi)型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
規(guī)格：0.5ml Designations: SC-1
Depositors: ?Cetus Corp.
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Cell Type: B lymphocyte; Epstein-Barr virus (EBV) transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,7.3
D13S317: 11,12
D16S539: 10,11
D5S818: 11,12
D7S820: 8,10
THO1: 7
TPOX: 6,9
vWA: 14
Comments:The line is homozygous for the sickle cell allele at the hemoglobin beta chain locus.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp5 and 1.5 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 92.5%; DMSO, 7.5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 21913: Mullis KB, et al. Process for amplifying, detecting, and/or-cloning nucleic acid sequences. US Patent 4,683,195 dated Jul 28 1987
21914: Mullis KB. Process for amplifying nucleic acid sequences. US Patent 4,683,202 dated Jul 28 1987
數(shù)量：大量
細(xì)胞形態(tài)：淋巴樣
ATCC Number：CRL-8756?
運(yùn)輸方式：凍存運(yùn)輸
細(xì)胞類(lèi)型：B淋巴細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：人
規(guī)格：0.5ml Designations: SC-1
Depositors: ?Cetus Corp.
Biosafety Level:2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Source: Cell Type: B lymphocyte; Epstein-Barr virus (EBV) transformed
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,7.3
D13S317: 11,12
D16S539: 10,11
D5S818: 11,12
D7S820: 8,10
THO1: 7
TPOX: 6,9
vWA: 14
Comments:The line is homozygous for the sickle cell allele at the hemoglobin beta chain locus.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 3 to 4 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp5 viable cells/ml.
Maintain cell density between 5 X 10 exp5 and 1.5 X 10 exp6 viable cells/ml.
Preservation: Culture medium, 92.5%; DMSO, 7.5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 21913: Mullis KB, et al. Process for amplifying, detecting, and/or-cloning nucleic acid sequences. US Patent 4,683,195 dated Jul 28 1987
21914: Mullis KB. Process for amplifying nucleic acid sequences. US Patent 4,683,202 dated Jul 28 1987
