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組織來(lái)源：tubule
ATCC Number：CRL-2762?
細(xì)胞形態(tài)：上皮樣
運(yùn)輸方式：凍存運(yùn)輸
器官來(lái)源：腎臟
數(shù)量：大量
生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)
細(xì)胞類型：上皮細(xì)胞
是否是腫瘤細(xì)胞：0
物種來(lái)源：其他
年限：1 day newborn
規(guī)格：1ml Designations: SQMK-FP
Depositors: ?JG Scammell
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:adherent
Organism: Saimiri boliviensis boliviensis
Morphology:epithelial


Source: Organ: kidney
Tissue: tubule
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions:This material is not available for international distribution due to the lack of historical data to support the issuance of a U.S. Fish and Wildlife Service Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES) export permit
Isolation: Isolation date: 1997
Cytogenetic Analysis:hyperdiploid with chromosome numbers ranging from 52 to 57 (submetacentric chromosome pair 15 and acrocentric chromosome pair 16). [61330 ]
Age: 1 day newborn
Gender: male
Comments:SQMK-FP is a continuous, tubular, kidney epithelial cell line derived from a newborn squirrel monkey. Normal kidney tissue was minced and cultured. Proliferating cells were subsequently cloned by the limiting dilution. SQMK-FP cells have cell junctions with radiating filaments, indicating desmosomes and numerous surface projections containing longitudinally oriented filaments typical of tubular epithelium. The cells exhibit elevated expression of the FK506-binding immunophilin FKBP51. FKBP51 is the common cause of glucocorticoid resistance typical of the squirrel monkey.The cell line can be used to study the biology of the squirrel monkey in vitro.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
   An inoculum of 5 X 10(4) to 1 X 10(5) viable cells/cm2 is recommended. Do not exceed 3 X 10(6) cells/cm2.
6. Incubate cultures at 37?C.

Interval: Maintain cultures at a cell concentration between 1 X 10(5) and 2 X 10(6) cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every two to three days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: approximately 22 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 61330: Scammell JG, et al. A kidney epithelial cell line from a bolivian squirrel monkey. In Vitro Cell. Dev. Biol. Anim. 38: 258-261, 2002. PubMed: 12418921