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平臺(tái)編號(hào)：bio-68553
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國際編號(hào)：CRL-3003?
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細(xì)胞信息： JVM-13
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費(fèi)用：
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注意事項(xiàng)：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動(dòng)物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
JVM-13
生長狀態(tài)：懸浮生長
數(shù)量：大量
年限：late 60's
器官來源：外周血
運(yùn)輸方式：凍存運(yùn)輸
ATCC Number：CRL-3003?
相關(guān)疾病：其他疾病
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：人
細(xì)胞形態(tài)：淋巴樣
規(guī)格：0.5mg Designations: JVM-13
Depositors: ?J.V.Melo
Biosafety Level:2
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens
Morphology:lymphoblast
Characterization Data
Source: Organ: peripheral blood
Cell type: lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transformed
Disease: B-prolymphocytic leukemia (B-PLL)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression:CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7+ (verified at ATCC )
DNA Profile (STR):Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18
Cytogenetic Analysis:Modal chromosome number: 47This is a pseudodiploid human cell line of male origin with a modal chromosome number of 47. Most of the cells contain the derivative chromosomes: add(2q), t(8;13)(p12;q13) and t(8;?)(p12;?). Overall, the karyology was consistent with other published data [PubMed: 3262465].
Age: late 60's
Gender: male
Ethnicity: Caucasian
Comments:Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0℃
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 x 10(4) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Preservation: 70% growth medium; 20% FBS; 10% DMSO
Doubling Time: about 58 hours
References: 92892: Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393
92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
