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平臺編號：bio-68554
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國際編號：CRL-2627?
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細(xì)胞信息： GDM-1
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規(guī)格：frozen
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用途：ATCC原裝細(xì)胞
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服務(wù)費用：
加載中…… - 訂購
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注意事項：僅用于科學(xué)研究或者工業(yè)應(yīng)用等非醫(yī)療目的不可用于人類或動物的臨床診斷或治療，非藥用，非食用（產(chǎn)品信息以出庫為準(zhǔn)）
GDM-1
生長狀態(tài)：懸浮生長
器官來源：外周血
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：人
運(yùn)輸方式：凍存運(yùn)輸
年限：66 - 67 years
ATCC Number：CRL-2627?
數(shù)量：大量
相關(guān)疾病：其他疾病
細(xì)胞形態(tài)：淋巴樣
規(guī)格：0.5mg Designations: GDM-1
Depositors: ?H Ben-Bassat
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens deposited as human
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: myelomonoblastic leukemia
Cell Type: monoblast;
Cellular Products:lysozyme [53284 ]
myeloperoxidase [53284 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:complement (C3) [53284 ]
Fc [53284 ]
Tumorigenic:No
Antigen Expression:Ia +; myeloid leukemia antigen (M-1) [53284 ]
Age: 66 - 67 years
Gender: female
Ethnicity: White
Comments:The GDM-1 cell line was established in 1980 from the peripheral blood of a patient with a Philadelphia chromosome negative myeloproliferative disorder, after transformation to acute myelomonoblastic leukemia. [53284 ]
The cells lack B- and T-cell surface markers including T-associated antigens, E-rosetting capacity, surface and intracytoplasmic immunoglobulins. They are non-specific esterase positive. [53284 ]
The cells are phagocytic for latex beads and iron particles. Exposure to phorbol 12-myristate 13-acetate (TPA) increases the phagocytic activity. TPA also induces macrophage-like differentiation. [53284 ]
The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-). [53284 ]
This cell line is a model system for studying myelomonocytic disorders and leukemias.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Doubling Time: 24 to 36 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53284: Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552
器官來源：外周血
細(xì)胞類型：其他細(xì)胞類型
是否是腫瘤細(xì)胞：0
物種來源：人
運(yùn)輸方式：凍存運(yùn)輸
年限：66 - 67 years
ATCC Number：CRL-2627?
數(shù)量：大量
相關(guān)疾病：其他疾病
細(xì)胞形態(tài)：淋巴樣
規(guī)格：0.5mg Designations: GDM-1
Depositors: ?H Ben-Bassat
Biosafety Level:1
Shipped: frozen
Medium & Serum: See Propagation Growth Properties:suspension
Organism: Homo sapiens deposited as human
Morphology:lymphoblast
Source: Organ: peripheral blood
Disease: myelomonoblastic leukemia
Cell Type: monoblast;
Cellular Products:lysozyme [53284 ]
myeloperoxidase [53284 ]
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:complement (C3) [53284 ]
Fc [53284 ]
Tumorigenic:No
Antigen Expression:Ia +; myeloid leukemia antigen (M-1) [53284 ]
Age: 66 - 67 years
Gender: female
Ethnicity: White
Comments:The GDM-1 cell line was established in 1980 from the peripheral blood of a patient with a Philadelphia chromosome negative myeloproliferative disorder, after transformation to acute myelomonoblastic leukemia. [53284 ]
The cells lack B- and T-cell surface markers including T-associated antigens, E-rosetting capacity, surface and intracytoplasmic immunoglobulins. They are non-specific esterase positive. [53284 ]
The cells are phagocytic for latex beads and iron particles. Exposure to phorbol 12-myristate 13-acetate (TPA) increases the phagocytic activity. TPA also induces macrophage-like differentiation. [53284 ]
The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-). [53284 ]
This cell line is a model system for studying myelomonocytic disorders and leukemias.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Doubling Time: 24 to 36 hrs
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53284: Ben-Bassat H, et al. Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552
